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GCSH Polyclonal antibody

GCSH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16726-1-AP

Synonyms

GCE, GCSH, NKH



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human liver tissue, mouse brain tissue, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue, human testis tissue, rat ovary tissue, human skin tissue, human liver tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

16726-1-AP targets GCSH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCSH fusion protein Ag10174
Full Name glycine cleavage system protein H (aminomethyl carrier)
Calculated molecular weight 19 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC000790
Gene symbol GCSH
Gene ID (NCBI) 2653
RRIDAB_2247351
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encoding a precursor protein of 173 amino acids and a mature protein of 125 amino acids.The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).

Protocols

Product Specific Protocols
WB protocol for GCSH antibody 16726-1-APDownload protocol
IHC protocol for GCSH antibody 16726-1-APDownload protocol
IF protocol for GCSH antibody 16726-1-APDownload protocol
IP protocol for GCSH antibody 16726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Science

Copper induces cell death by targeting lipoylated TCA cycle proteins.

Authors - Peter Tsvetkov
humanWB

Nat Commun

Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.

Authors - Jiliang Xia
  • KD Validated
humanWB

J Biol Chem

Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.

Authors - Daniel R Crooks
humanWB,IHC,IF

Sci Rep

GCSH antisense regulation determines breast cancer cells' viability.

Authors - Anna Adamus
humanWB

J Biol Chem

FDX1 regulates cellular protein lipoylation through direct binding to LIAS

Authors - Margaret B Dreishpoon
humanWB

PLoS One

Amino Acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.

Authors - Mark A Johnson