|Positive WB detected in||HEK-293 cells, HeLa cells, human liver tissue, mouse brain tissue, mouse kidney tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human kidney tissue, rat ovary tissue, human placenta tissue, human testis tissue, human skin tissue, human liver tissue, human ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16726-1-AP targets GCSH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GCSH fusion protein Ag10174|
|Full Name||glycine cleavage system protein H (aminomethyl carrier)|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||15 kDa|
|GenBank accession number||BC000790|
|Gene ID (NCBI)||2653|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encoding a precursor protein of 173 amino acids and a mature protein of 125 amino acids.The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).
|Product Specific Protocols|
|WB protocol for GCSH antibody 16726-1-AP||Download protocol|
|IHC protocol for GCSH antibody 16726-1-AP||Download protocol|
|IF protocol for GCSH antibody 16726-1-AP||Download protocol|
|IP protocol for GCSH antibody 16726-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Biol Chem
Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.
Life Sci Alliance
Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.
Amino Acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.
GCSH antisense regulation determines breast cancer cells' viability.