GCSH Polyclonal antibody

GCSH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16726-1-AP

Synonyms

GCE, GCSH, NKH



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human liver tissue, mouse brain tissue, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, rat ovary tissue, human placenta tissue, human testis tissue, human skin tissue, human liver tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16726-1-AP targets GCSH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCSH fusion protein Ag10174
Full Name glycine cleavage system protein H (aminomethyl carrier)
Calculated molecular weight 19 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC000790
Gene symbol GCSH
Gene ID (NCBI) 2653
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encoding a precursor protein of 173 amino acids and a mature protein of 125 amino acids.The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).

Protocols

Product Specific Protocols
WB protocol for GCSH antibody 16726-1-APDownload protocol
IHC protocol for GCSH antibody 16726-1-APDownload protocol
IF protocol for GCSH antibody 16726-1-APDownload protocol
IP protocol for GCSH antibody 16726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.

Authors - Daniel R Crooks
mouseWB

Life Sci Alliance

Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.

Authors - Shengya Tian
humanWB

PLoS One

Amino Acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.

Authors - Mark A Johnson
humanWB,IHC,IF

Sci Rep

GCSH antisense regulation determines breast cancer cells' viability.

Authors - Anna Adamus