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WB analysis using 13152-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 13152-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of mouse brain using 13152-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of human brain using 13152-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13152-1-AP (GDAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of rat brain using 13152-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 13152-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13152-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13152-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13152-1-AP (GDAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GDAP1 Polyclonal antibody

Name: GDAP1 Polyclonal antibody
Brand: Proteintech
SKU: 13152-1-AP

GDAP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13152-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CMTRIA, Ganglioside-induced differentiation-associated protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, human brain tissue, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected in	rat brain tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13152-1-AP targets GDAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3769 Product name: Recombinant human GDAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-257 aa of BC024939 Sequence: MRLNSTGEVPVLIHGENIICEATQIIDYLEQTFLDERTPRLMPDKESMYYPRVQHYRELLDSLPMDAYTHGCILHPELTVDSMIPAYATTRIRSQIGNTESELKKLAEENPDLQEAYIAKQKRLKSKLLDHDNVKYLKKILDELEKVLDQVETELQRRNEETPEEGQQPWLCGESFTLADVSLAVTLHRLKFLGFARRNWGNGKRPNLETYYERVLKRKTFNKVLGHVNNILISAVLPTAFRVAKKRAPKVLGTTLV Predict reactive species
Full Name	ganglioside-induced differentiation-associated protein 1
Calculated Molecular Weight	358 aa, 41 kDa
Observed Molecular Weight	36-41 kDa
GenBank Accession Number	BC024939
Gene Symbol	GDAP1
Gene ID (NCBI)	54332
RRID	AB_2232103
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TB36
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.