GDE1 Polyclonal antibody

GDE1 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11272-2-AP

Synonyms

363E6.2, GDE1, MIR16

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 11272-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11272-2-AP (GDE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11272-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11272-2-AP (GDE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11272-2-AP targets GDE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDE1 fusion protein Ag1821
• Full Name: glycerophosphodiester phosphodiesterase 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 55-66 kDa
• GenBank Accession Number: BC014981
• Gene Symbol: GDE1
• Gene ID (NCBI): 51573
• RRID: AB_2247628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDE1(glycerophosphodiester phosphodiesterase 1) is also named as MIR16 and belongs to the glycerophosphoryl diester phosphodiesterase family. It is an 43 kDa integral membrane glycoprotein, which remains associated with membranes after alkaline (pH 11) extraction and is sensitive to endoglycosidase H and most likely involved in the remodeling of membrane PIs and regulation of membrane phospholipid composition, and hence in intracellular membrane trafficking, as well as in the modulation of G proteinmediated signaling(PMID:12576545). The full length protein has two glycosylation sites.

Publications

Species	Application	Title
human	WB	Invest Ophthalmol Vis Sci; Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves.; Authors - Sasha M Milbeck