GDF10 Monoclonal antibody

• 

  WB analysis of pig bone marrow using 66371-1-Ig

  pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66371-1-Ig (GDF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66371-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66371-1-Ig (GDF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GDF10 Monoclonal Antibody for WB, ELISA

Cat No. 66371-1-Ig

Clone No.1B7D12

Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB, ELISA

Synonyms

BIP, BMP 3b, BMP3B, Bone inducing protein, Bone morphogenetic protein 3B, GDF 10, GDF10

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Tested Applications

• Positive WB detected in: pig bone marrow tissue, ROS1728 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66371-1-Ig targets GDF10 in WB, ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4906

  Product name: Recombinant human GDF10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-288 aa of BC028237

  Sequence: MALAPPPRGLWQAKDISPIVKAARRDGELLLSAQLDSEERDPGVPRPSPYAPYILVYANDLAISEPNSVAVTLQRYDPFPAGDPEPRAAPNNSADPRVRRAAQATGPLQDNELPGLDERPPRAHAQHFHKHQLWPSPFRALKPRPGRKDRRKKGQEVFMAASQVLDFDEKTMQKARRKQWDEPRVCSRRYLKVDFADIGWNEWIISPKSFDAYYCAGACEFPMPKIVRPSNHATIQSIVRAVGIIPGIPEPCCVPDKMNSLGVLFLDENRNVVLKVYPNMSVDTCACR

• Full Name: growth differentiation factor 10
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC028237
• Gene Symbol: GDF10
• Gene ID (NCBI): 2662
• RRID: AB_2881750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55107
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth differentiation factor-10 (GDF10), also known as BMP3b, is a member of the transforming growth factor-b (TGF-b) superfamily. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This promotes neural repair after stroke. GDF10 plays a role in head formation and may have multiple roles in skeletal morphogenesis. Additionally, GDF10 may act as a tumor suppressor and reduced expression of this gene is associated with oral cancer.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Brain Behav Immun; Schisantherin A attenuates ischemia/reperfusion-induced neuronal injury in rats via regulation of TLR4 and C5aR1 signaling pathways.; Authors - Yun Wei Shi