GDF10 Monoclonal antibody

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• GDF10 Monoclonal antibody (66371-1-Ig)

GDF10 Monoclonal Antibody for WB, ELISA

Cat No. 66371-1-Ig Copied!

Clone No. 1B7D12

Publications (1)

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Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB, ELISA

Synonyms

BIP, BMP 3b, BMP3B, Bone inducing protein, Bone morphogenetic protein 3B, GDF 10, GDF10

• 

  WB analysis of pig bone marrow using 66371-1-Ig

  pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66371-1-Ig (GDF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of ROS1728 using 66371-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66371-1-Ig (GDF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: pig bone marrow tissue, ROS1728 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66371-1-Ig targets GDF10 in WB, ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4906

  Product name: Recombinant human GDF10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-288 aa of BC028237

  Sequence: MALAPPPRGLWQAKDISPIVKAARRDGELLLSAQLDSEERDPGVPRPSPYAPYILVYANDLAISEPNSVAVTLQRYDPFPAGDPEPRAAPNNSADPRVRRAAQATGPLQDNELPGLDERPPRAHAQHFHKHQLWPSPFRALKPRPGRKDRRKKGQEVFMAASQVLDFDEKTMQKARRKQWDEPRVCSRRYLKVDFADIGWNEWIISPKSFDAYYCAGACEFPMPKIVRPSNHATIQSIVRAVGIIPGIPEPCCVPDKMNSLGVLFLDENRNVVLKVYPNMSVDTCACR

• Full Name: growth differentiation factor 10
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC028237
• Gene Symbol: GDF10
• Gene ID (NCBI): 2662
• RRID: AB_2881750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55107
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth differentiation factor-10 (GDF10), also known as BMP3b, is a member of the transforming growth factor-b (TGF-b) superfamily. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This promotes neural repair after stroke. GDF10 plays a role in head formation and may have multiple roles in skeletal morphogenesis. Additionally, GDF10 may act as a tumor suppressor and reduced expression of this gene is associated with oral cancer.

Publications

What published studies show

The GDF10 Monoclonal antibody (1B7D12), catalog number 66371-1-Ig, has 1 total citation. It was cited in Brain, Behavior, and Immunity (IF 7.5), in a study investigating Schisantherin A's attenuation of ischemia/reperfusion-induced neuronal injury in rats via TLR4 and C5aR1 signaling pathways. The citation is associated with the research fields of neurology and neuroinflammation, with the antibody used for Western blot in rat samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Brain Behav Immun; Schisantherin A attenuates ischemia/reperfusion-induced neuronal injury in rats via regulation of TLR4 and C5aR1 signaling pathways.; Authors - Yun Wei Shi