GDF5 Recombinant monoclonal antibody, PBS Only

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  IF Staining of RAW 264.7 using 83988-4-RR (same clone as 83988-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using GDF5 antibody (83988-4-RR, Clone: 240944E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83988-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83988-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83988-4-RR against Human GDF5 were performed. The affinity constant is 4.07 nM.

GDF5 Uni-rAb^® Recombinant Antibody for IF/ICC, ELISA

Cat No. 83988-4-PBS

Clone No.240944E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, ELISA

Synonyms

GDF-5, GDF 5, CDMP-1, Cartilage-derived morphogenetic protein 1, Bone morphogenetic protein 14

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Product Information

83988-4-PBS targets GDF5 in IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6238

  Product name: Recombinant human GDF5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 143-501 aa of BC032495

  Sequence: SFLLKKAREPGPPREPKEPFRPPPITPHEYMLSLYRTLSDADRKGGNSSVKLEAGLANTITSFIDKGQDDRGPVVRKQRYVFDISALEKDGLLGAELRILRKKPSDTAKPAAPGGGRAAQLKLSSCPSGRQPASLLDVRSVPGLDGSGWEVFDIWKLFRNFKNSAQLCLELEAWERGRAVDLRGLGFDRAARQVHEKALFLVFGRTKKRDLFFNEIKARSGQDDKTVYEYLFSQRRKRRAPLATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR

• Full Name: growth differentiation factor 5
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC032495
• Gene Symbol: GDF5
• Gene ID (NCBI): 8200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P43026
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Growth and Differentiation Factor 5 (GDF5) plays important roles during joint formation. It is one of the earliest genes expressed in the embryonic joint interzone7-10, fated to give rise to joint tissues including articular cartilage, synovium, menisci, and ligaments.