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GDI2 Polyclonal antibody

GDI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 10116-1-AP


GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, Rab GDI β, RABGDIB

Tested Applications

Positive WB detected inA431 cells, rat brain tissue, mouse spleen tissue, rat spleen tissue, HeLa cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10116-1-AP targets GDI2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, yeast, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GDI2 fusion protein Ag0170
Full Name GDP dissociation inhibitor 2
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC005145
Gene Symbol GDI2
Gene ID (NCBI) 2665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.


Product Specific Protocols
WB protocol for GDI2 antibody 10116-1-APDownload protocol
IHC protocol for GDI2 antibody 10116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




A bifunctional bacterial protein links GDI displacement to Rab1 activation.

Authors - Machner Matthias P MP

Nat Chem Biol

Enhanced mapping of small-molecule binding sites in cells

Authors - Jacob M Wozniak

Proc Natl Acad Sci U S A

Structural mechanism of host Rab1 activation by the bifunctional Legionella type IV effector SidM/DrrA.

Authors - Zhu Yongqun Y

Curr Biol

Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.

Authors - Anil Kumar Ganga

Hum Mol Genet

Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.

Authors - McCray Brett A BA

Mol Cell Proteomics

Differential proteomics analysis of synaptic proteins identifies potential cellular targets and protein mediators of synaptic neuroprotection conferred by the slow Wallerian degeneration (Wlds) gene.

Authors - Wishart Thomas M TM


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Guo-rong (Verified Customer) (08-30-2022)

Specific and clear results

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: ARPE-19
GDI2 Antibody Western Blot validation (1:2000 dilution) in ARPE-19 (Cat no:10116-1-AP)