|Positive WB detected in||rat brain tissue, HeLa cells, mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, human pancreas cancer tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10116-1-AP targets GDI2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse, yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GDI2 fusion protein Ag0170|
|Full Name||GDP dissociation inhibitor 2|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC005145|
|Gene ID (NCBI)||2665|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.
Biochim Biophys Acta
Serum proteomic-based analysis of pancreatic carcinoma for the identification of potential cancer biomarkers.
Cell Death Dis
TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.
Bioinformatics analysis of Rab GDP dissociation inhibitor beta and its expression in non-small cell lung cancer.
Cancer Biol Ther
SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome.
Int J Oncol
Proteomics-based identification of a group of apoptosis-related proteins and biomarkers in gastric cancer.
Levels of the Rab GDP dissociation inhibitor (GDI) are altered in the prenatal restrain stress mouse model of schizophrenia and are differentially regulated by the mGlu2/3 receptor agonists, LY379268 and LY354740.