GDI2 Polyclonal antibody

GDI2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10116-1-AP

Synonyms

GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, Rab GDI β, RABGDIB



Tested Applications

Positive WB detected inrat brain tissue, HeLa cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue, human pancreas cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10116-1-AP targets GDI2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GDI2 fusion protein Ag0170
Full Name GDP dissociation inhibitor 2
Calculated molecular weight 47 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC005145
Gene symbol GDI2
Gene ID (NCBI) 2665
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.

Protocols

Product Specific Protocols
WB protocol for GDI2 antibody 10116-1-APDownload protocol
IHC protocol for GDI2 antibody 10116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biochim Biophys Acta

Serum proteomic-based analysis of pancreatic carcinoma for the identification of potential cancer biomarkers.

Authors - Sun Zhi-Ling ZL
WB

Cell Death Dis

TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.

Authors - Jia Hu
humanIHC

Diagn Pathol

Bioinformatics analysis of Rab GDP dissociation inhibitor beta and its expression in non-small cell lung cancer.

Authors - Zongjuan Ming
humanIHC

Cancer Biol Ther

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome.

Authors - Kashyap Manoj Kumar MK
humanWB

Int J Oncol

Proteomics-based identification of a group of apoptosis-related proteins and biomarkers in gastric cancer.

Authors - Zhigang Bai
mouseWB

Neuropharmacology

Levels of the Rab GDP dissociation inhibitor (GDI) are altered in the prenatal restrain stress mouse model of schizophrenia and are differentially regulated by the mGlu2/3 receptor agonists, LY379268 and LY354740.

Authors - Rosamaria Orlando