GDPD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GDPD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse , Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27861-1-AP

Synonyms

GDE4, GDPD1, UGPQ

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Validation Data Gallery

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  WB analysis using 27861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27861-1-AP (GDPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 27861-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27861-1-AP (GDPD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 27861-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27861-1-AP (GDPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse ovary tissue, rat testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

27861-1-AP targets GDPD1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

• Tested Reactivity: Human, Mouse , Rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDPD1 fusion protein Ag27371
• Full Name: glycerophosphodiester phosphodiesterase domain containing 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 33-36 kDa
• GenBank Accession Number: BC034432
• Gene Symbol: GDPD1
• Gene ID (NCBI): 284161
• RRID: AB_2880996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cell Death Differ; A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.; Authors - Xiaoyu Qi
human	WB	J Lipid Res; Development of a selective fluorescence-based enzyme assay for glycerophosphodiesterase family members GDE4 and GDE7; Authors - Keisuke Kitakaze ; KO Validated