GET4 Polyclonal antibody

GET4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27768-1-AP

Publications (2)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

C7orf20, CEE, CGI 20, CGI-20, Conserved edge-expressed protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 27768-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27768-1-AP (GET4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 27768-1-AP
HEK-293 cells and MCF-7 cells were subjected to SDS PAGE followed by western blot with 27768-1-AP (GET4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 27768-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27768-1-AP (GET4 antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 27768-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27768-1-AP (GET4 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 27768-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27768-1-AP (GET4 antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 27768-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27768-1-AP (GET4 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, MCF-7 cells, HeLa cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27768-1-AP targets GET4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26904 Product name: Recombinant human C7orf20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 5-141 aa of BC003550 Sequence: AAAPNPADGTPKVLLLSGQPASAAGAPAGQALPLMVPAQRGASPEAASGGLPQARKRQRLTHLSPEEKALRRKLKNRVAAQTARDRKKARMSELEQQVVDLEEENQKLLLENQLLREKTHGLVVENQELRQRLGMDA Predict reactive species
Full Name	chromosome 7 open reading frame 20
Calculated Molecular Weight	327 aa, 37 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	BC003550
Gene Symbol	GET4
Gene ID (NCBI)	51608
RRID	AB_2880967
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7L5D6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for GET4 antibody 27768-1-AP	Download protocol
WB protocol for GET4 antibody 27768-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The GET4 polyclonal antibody (catalog number 27768-1-AP) has 2 total citations. These appear in Nature Cell Biology and EMBO Reports. The associated research fields include ribosome-mediated translation of transcripts related to spinal muscular atrophy, and protein quality control involving UBR5-mediated heterotypic ubiquitin chains.

Species	Application	Title
mouse	WB	Nat Cell Biol SMN-primed ribosomes modulate the translation of transcripts related to spinal muscular atrophy. Authors - Fabio Lauria View Article
human	WB	EMBO Rep C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains Authors - Julia Jülg View Article

Reviews

4.0 out of 5

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1 verified customer reviews

What customers say

The single review for this product reports a Western Blot application on mouse heart tissue, rated 4 out of 5. The user observed a band at the right size with moderate background. Overall, the feedback suggests the antibody performs adequately with correct molecular weight detection, though some background was noted.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

WEI (Verified Customer) (03-08-2022)

Band at right size with moderate background.