Cat no : gb2AF568
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
|Applications||IF, IHC, ICC|
|Recommended Dilution||IF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000|
|Affinity||Dissociation constant KD of 1 pM|
|Specificity/Target||AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP|
|Conjugate||Alexa Fluor® 568|
|Storage Buffer||10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide|
|Storage Condition||Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.|
|SDS GFP-Booster Alexa Fluor 568 (PDF)|
|Protocol GFP-Booster Alexa Fluor 568 (PDF)|
|Fluorescent protein specificity table Nano-Booster|
|Chromotek nanobodies brochure (PDF)|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Roxk (Verified Customer) (07-22-2022)
this is the second time that I purchase the GFP booster, first time i used the 488 and it worked great, this time I got the 568. Tissue used is colon and the two BoosterGFP worked differently, with the 568 having lots of trouble (lot issue???)