ChromoTek GFP-Booster Alexa Fluor® 568
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
Specificity
CFP, eGFP, AcGFP, eYFP
Applications
IF, IHC, ICC
Conjugate
Alexa Fluor® 568
Cat no : gb2AF568
Synonyms
Validation Data Gallery
Product Information
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
Applications | IF, IHC, ICC |
Recommended Dilution | IF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000 |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 1 pM |
Specificity/Target | AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP |
Conjugate | Alexa Fluor® 568 |
Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide |
Storage Condition | Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months. |
Documentation
SDS |
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SDS GFP-Booster Alexa Fluor 568 (PDF) |
Protocol |
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Protocol GFP-Booster Alexa Fluor 568 (PDF) |
GFP-TRAP Specificity |
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Fluorescent protein specificity table Nano-Booster |
Brochure |
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Chromotek nanobodies brochure (PDF) |
Publications
Application | Title |
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Nat Commun Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Roxk (Verified Customer) (07-22-2022) | this is the second time that I purchase the GFP booster, first time i used the 488 and it worked great, this time I got the 568. Tissue used is colon and the two BoosterGFP worked differently, with the 568 having lots of trouble (lot issue???)
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