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ChromoTek GFP-Booster Alexa Fluor® 568

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Specificity

CFP, eGFP, AcGFP, eYFP

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 568

Cat no : gb2AF568

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

Validation Data Gallery

  • Confocal images of HeLa cells transiently transfected with MannosidaseII-GFP (green) and immunostained with GFP-Booster Alexa Fluor 568 (red). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Confocal images of HeLa cells transiently transfected with MannosidaseII-GFP (green) and immunostained with GFP-Booster Alexa Fluor 568 (red). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

ApplicationsIF, IHC, ICC
Recommended DilutionIF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 1 pM
Specificity/TargetAcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP
ConjugateAlexa Fluor® 568
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.

Publications

ApplicationTitle
IF

Nat Commun

Poly(ADP-ribose) binding to Chk1 at stalled replication forks is required for S-phase checkpoint activation.

Authors - WooKee Min
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Roxk (Verified Customer) (07-22-2022)

this is the second time that I purchase the GFP booster, first time i used the 488 and it worked great, this time I got the 568. Tissue used is colon and the two BoosterGFP worked differently, with the 568 having lots of trouble (lot issue???)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Mouse colon