Cat no : gb2AF647
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
|Description||The GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:
• Considerably higher tissue penetration rates
• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments
• Less than 2 nm epitope-label displacement minimizes linkage error
• Monovalent VHHs do not cluster their epitopes
• Validation: structure and function characterized
• Consistent and reliable performance due to recombinant production
|Applications||IF, IHC, ICC|
|Specificity/Target||CFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet|
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
|Conjugate||Alexa Fluor® 647|
|Suggested Dilution||IF/ICC/IHC: 1:500- 1:1,000|
|DOL||2 fluorophores per Nanobody|
|Affinity (KD)||Dissociation constant KD of 1 pM|
|Storage Buffer||10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide|
|Storage Condition||Shipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.|
|Note||This product is for research use only, not for diagnostic or therapeutic use|
|SDS GFP-Booster Alexa Fluor 647 (PDF)|
|Protocol GFP-Booster Alexa Fluor 647 (PDF)|
|Fluorescent protein specificity table Nano-Booster|
|Chromotek nanobodies brochure (PDF)|
Varying label density allows artifact-free analysis of membrane-protein nanoclusters.
Molecular organization and mechanics of single vimentin filaments revealed by super-resolution imaging.
Extent of myosin penetration within the actin cortex regulates cell surface mechanics.
The nanoscale architecture of force-bearing focal adhesions.
Chromatin stiffening underlies enhanced locus mobility after DNA damage in budding yeast.
DRP1 interacts directly with BAX to induce its activation and apoptosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Andrea (Verified Customer) (09-19-2023)
Very good signal enhancement.