ChromoTek GFP-Booster Alexa Fluor® 647
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
Ready to use reagent
Specificity
CFP, eGFP, AcGFP, eYFP
Applications
IF, IHC, ICC
Conjugate
Alexa Fluor® 647
Cat no : gb2AF647
Synonyms
Validation Data Gallery
Product Information
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
Applications | IF, IHC, ICC |
Recommended Dilution | IF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000 |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 1 pM |
Specificity/Target | AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP |
Conjugate | Alexa Fluor® 647 |
Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide |
Storage Condition | Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months. |
Documentation
SDS |
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SDS GFP-Booster Alexa Fluor 647 (PDF) |
Protocol |
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Protocol GFP-Booster Alexa Fluor 647 (PDF) |
Brochure |
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Product brochure (PDF) |
Publications
Application | Title |
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Methods Appl Fluoresc Spatial filter and its application in three-dimensional single molecule localization microscopy. | |
Front Physiol Modulation of Calcium Transients in Cardiomyocytes by Transient Receptor Potential Canonical 6 Channels. | |
Mol Biol Cell Spatial cues and not spindle pole maturation drive the asymmetry of astral microtubules between new and preexisting spindle poles. | |
Elife Direct comparison of clathrin-mediated endocytosis in budding and fission yeast reveals conserved and evolvable features. |