ChromoTek GFP-Booster Alexa Fluor® 647

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Ready to use reagent

Specificity

CFP, eGFP, AcGFP, eYFP

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 647

Cat no : gb2AF647

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

ApplicationsIF, IHC, ICC
Recommended DilutionIF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000
Clonality Monoclonal
Host Alpaca
AffinityDissociation constant KD of 1 pM
Specificity/TargetAcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP
ConjugateAlexa Fluor® 647
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.

Publications

ApplicationTitle
IF

PLoS Genet

Sensors at centrosomes reveal determinants of local separase activity.

Authors - Fikret Gurkan Agircan
IF

Methods Appl Fluoresc

Spatial filter and its application in three-dimensional single molecule localization microscopy.

Authors - Xiaoming Fan
IF

Front Physiol

Modulation of Calcium Transients in Cardiomyocytes by Transient Receptor Potential Canonical 6 Channels.

Authors - Azmi A Ahmad
IF

Mol Biol Cell

Spatial cues and not spindle pole maturation drive the asymmetry of astral microtubules between new and preexisting spindle poles.

Authors - Jette Lengefeld
IF

Sci Signal

TNFR1 membrane reorganization promotes distinct modes of TNFα signaling.

Authors - Penny E Morton
IF

Elife

Direct comparison of clathrin-mediated endocytosis in budding and fission yeast reveals conserved and evolvable features.

Authors - Yidi Sun