ChromoTek GFP-Booster Alexa Fluor® 647

Validation Data Gallery

• 

  Immunostaining of Tubulin-GFP: HeLa cells low expressing Tubulin-GFP. Top: GFP signal of Tubulin-GFP (green) and DAPI stain (blue); Bottom: Tubulin-GFP detection by GFP-Booster coupled to Alexa Fluor 647.

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Cat No. gb2AF647

Publications(32)

Reviews (1)

Specificity

GFP and GFP derivates

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 647

Host

Alpaca

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

---

Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

• Description: The GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

  • Considerably higher tissue penetration rates

  • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

  • Less than 2 nm epitope-label displacement minimizes linkage error

  • Monovalent VHHs do not cluster their epitopes

  • Validation: structure and function characterized

  • Consistent and reliable performance due to recombinant production

• Applications: IF, IHC, ICC
• Host: Alpaca
• Specificity/Target: CFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet; For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
• Conjugate: Alexa Fluor® 647
• Physical State: Liquid
• Suggested Dilution: IF/ICC/IHC: 1:500- 1:1,000
• Type: Nanobody
• Class: Recombinant
• DOL: 2 fluorophores per Nanobody
• Format: Alpaca single domain antibody, Nanobody or VHH; monovalent
• RRID: AB_2827575
• Affinity (KD): Dissociation constant K_D of 1 pM
• Excitation/ Emission: Excitation max: 650 nm, Emission max: 665 nm
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
• Size: 10 μL; 50 μL
• Note: This product is for research use only, not for diagnostic or therapeutic use

---

Publications

Application	Title
IF	Nat Methods; Varying label density allows artifact-free analysis of membrane-protein nanoclusters.; Authors - Florian Baumgart
IF	Sci Adv; Molecular organization and mechanics of single vimentin filaments revealed by super-resolution imaging.; Authors - Filipe Nunes Vicente
	Nat Commun; Extent of myosin penetration within the actin cortex regulates cell surface mechanics.; Authors - Binh An Truong Quang
IF	Nano Lett; The nanoscale architecture of force-bearing focal adhesions.; Authors - Hedde van Hoorn
IF	EMBO J; Chromatin stiffening underlies enhanced locus mobility after DNA damage in budding yeast.; Authors - Sébastien Herbert
IF	EMBO J; DRP1 interacts directly with BAX to induce its activation and apoptosis.; Authors - Andreas Jenner

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Andrea (Verified Customer) (09-19-2023)

Very good signal enhancement.

• Applications: Western Blot,Immunohistochemistry