ChromoTek GFP-Booster Alexa Fluor® 647

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Ready to use reagent

Specificity

CFP, eGFP, AcGFP, eYFP

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 647

Publications

9

Cat no : gb2AF647

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Immunostaining of Tubulin-GFP: HeLa cells low expressing Tubulin-GFP. Top: GFP signal of Tubulin-GFP (green) and DAPI stain (blue); Bottom: Tubulin-GFP detection by GFP-Booster coupled to Alexa Fluor 647.

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Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

• Applications: IF, IHC, ICC
• Recommended Dilution: IF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP
• Conjugate: Alexa Fluor® 647
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.

Publications

Application	Title
IF	PLoS Genet; Sensors at centrosomes reveal determinants of local separase activity.; Authors - Fikret Gurkan Agircan
IF	Methods Appl Fluoresc; Spatial filter and its application in three-dimensional single molecule localization microscopy.; Authors - Xiaoming Fan
IF	Front Physiol; Modulation of Calcium Transients in Cardiomyocytes by Transient Receptor Potential Canonical 6 Channels.; Authors - Azmi A Ahmad
IF	Mol Biol Cell; Spatial cues and not spindle pole maturation drive the asymmetry of astral microtubules between new and preexisting spindle poles.; Authors - Jette Lengefeld
IF	Sci Signal; TNFR1 membrane reorganization promotes distinct modes of TNFα signaling.; Authors - Penny E Morton
IF	Elife; Direct comparison of clathrin-mediated endocytosis in budding and fission yeast reveals conserved and evolvable features.; Authors - Yidi Sun