ChromoTek GFP-Booster ATTO647N
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
Specificity
CFP, eGFP, AcGFP, eYFP
Applications
IF, IHC, ICC
Conjugate
ATTO647N
Cat no : gba647n
Synonyms
Validation Data Gallery
Product Information
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
| Applications | IF, IHC, ICC |
| Recommended Dilution | F/ICC/IHC: 1:200; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:50 to 1:1,600 |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 1 pM |
| Specificity/Target | AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP |
| Conjugate | 0.5 – 1 mg/mL (conjugates) |
| Storage Buffer | 1x PBS, 0.09% sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 6 month. Do not freeze. Protect from light. |
Documentation
| SDS |
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| SDS GFP-Booster ATTO647N (PDF) |
| Protocol |
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| Protocol GFP-Booster ATTO647N (PDF) |
| Brochure |
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| Product brochure (PDF) |
Publications
| Application | Title |
|---|---|
Front Synaptic Neurosci Control of Transmembrane Protein Diffusion within the Postsynaptic Density Assessed by Simultaneous Single-Molecule Tracking and Localization Microscopy. | |
Data Brief High-speed single molecule imaging datasets of membrane proteins in rat basophilic leukemia cells. | |
Int J Mol Sci Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy | |
Nano Res Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation. | |
Cell Rep Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior. | |
Phys Chem Chem Phys Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy. |