ChromoTek GFP-Booster ATTO647N

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Ready to use reagent

Specificity

CFP, eGFP, AcGFP, eYFP

Applications

IF, IHC, ICC

Conjugate

ATTO647N

Publications

28

Cat no : gba647n

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Enhancement of GFP signal with GFP-Booster Atto488: Comparison of signal intensity of a cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.

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Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

• Applications: IF, IHC, ICC
• Recommended Dilution: F/ICC/IHC: 1:200; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:50 to 1:1,600
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP
• Conjugate: ATTO647N
• Storage Buffer: 1x PBS, 0.09% sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 6 month. Do not freeze. Protect from light.

Publications

Application	Title
IF	Front Synaptic Neurosci; Control of Transmembrane Protein Diffusion within the Postsynaptic Density Assessed by Simultaneous Single-Molecule Tracking and Localization Microscopy.; Authors - Tuo P Li
IF	Data Brief; High-speed single molecule imaging datasets of membrane proteins in rat basophilic leukemia cells.; Authors - Hanieh Mazloom-Farsibaf
IF	Int J Mol Sci; Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy; Authors - Kimberly Cramer
IF	Histochem Cell Biol; Coordinate-based co-localization-mediated analysis of arrestin clustering upon stimulation of the C-C chemokine receptor 5 with RANTES/CCL5 analogues.; Authors - Laura Tarancón Díez
IF	Phys Chem Chem Phys; Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy.; Authors - Thorben Cordes
IF	Biophys J; A Simple and Powerful Analysis of Lateral Subdiffusion Using Single Particle Tracking.; Authors - Marianne Renner