ChromoTek GFP-Booster ATTO488

Validation Data Gallery

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  Enhancement of GFP signal with GFP-Booster Atto488: Comparison of signal intensity of a cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Cat No. gba488

Publications(215)

Reviews (2)

Specificity

GFP and GFP derivates

Applications

IF, IHC, ICC

Conjugate

ATTO 488

Host

Alpaca

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

• Description: The GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

  • Considerably higher tissue penetration rates

  • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

  • Less than 2 nm epitope-label displacement minimizes linkage error

  • Monovalent VHHs do not cluster their epitopes

  • Validation: structure and function characterized

  • Consistent and reliable performance due to recombinant production

• Applications: IF, IHC, ICC
• Host: Alpaca
• Specificity/Target: CFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet; For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
• Conjugate: ATTO 488
• Physical State: Liquid
• Suggested Dilution: IF/ICC/IHC: 1:200
• Format: Alpaca single domain antibody, Nanobody or VHH; monovalent
• Type: Nanobody
• Class: Recombinant
• RRID: AB_2631386
• Affinity (KD): Dissociation constant K_D of 1 pM
• Excitation/ Emission: Excitation max: 501 nm, Emission max: 523 nm
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.
• Size: 10 μL; 100 μL
• Note: This product is for research use only, not for diagnostic or therapeutic use

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Publications

Application	Title
IF	Nature; Effector T-cell trafficking between the leptomeninges and the cerebrospinal fluid.; Authors - Christian Schläger
IF	Nature; Stabilization of chromatin topology safeguards genome integrity.; Authors - Fena Ochs
IF	Science; Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.; Authors - James M Halstead
IF	Cell; Structural Mechanism for Cargo Recognition by the Retromer Complex.; Authors - María Lucas
IF	Cell; Dynamic Architecture of DNA Repair Complexes and the Synaptonemal Complex at Sites of Meiotic Recombination.; Authors - Alexander Woglar
IF	Cell; Oxidative Metabolism Drives Immortalization of Neural Stem Cells during Tumorigenesis.; Authors - François Bonnay

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (11-02-2023)

Used at 1:200 in 4% PFA fixed samples to enhance signal for a low expression level fusion protein. Really good increase in signal with minimal background/non-specific staining. Used succesfully at both 1 hour and 30 minute room temperature incubation

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HUVEC

FH

Muthuraj (Verified Customer) (03-03-2023)

It was good, works excellent

• Applications: Immunofluorescence