ChromoTek GFP-Booster ATTO488

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Ready to use reagent

Specificity

CFP, eGFP, AcGFP, eYFP

Applications

IF, IHC, ICC

Conjugate

ATTO488

Publications

135

Cat no : gba488

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Enhancement of GFP signal with GFP-Booster Atto488: Comparison of signal intensity of a cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.

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Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

• Applications: IF, IHC, ICC
• Recommended Dilution: F/ICC/IHC: 1:200; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:50 to 1:1,600
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP
• Conjugate: ATTO488
• Storage Buffer: 1x PBS, 0.09% sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 6 month. Do not freeze. Protect from light.

Publications

Application	Title
IF	Cell Rep; Trio Haploinsufficiency Causes Neurodevelopmental Disease-Associated Deficits.; Authors - Sara Marie Katrancha
IF	PLoS Genet; Scribbled Optimizes BMP Signaling through Its Receptor Internalization to the Rab5 Endosome and Promote Robust Epithelial Morphogenesis.; Authors - Jinghua Gui
IF	Nat Struct Mol Biol; E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis.; Authors - Leena Ackermann
IF	Biochim Biophys Acta; Interaction with epsin 1 regulates the constitutive clathrin-dependent internalization of ErbB3.; Authors - Monika Szymanska
IF	Cell Rep; 5-Hydroxymethylcytosine Marks Sites of DNA Damage and Promotes Genome Stability.; Authors - Georgia Rose Kafer
IF	Methods Mol Biol; Imaging of phenotypes, gene expression, and protein localization during embryonic root formation in Arabidopsis.; Authors - Cristina Llavata-Peris