ChromoTek GFP-Booster Alexa Fluor® 488

Validation Data Gallery

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  Confocal image of HeLa cells transiently transfected with Tom70-eGFP and immunostained with GFP-Booster Alexa Fluor 488 (green). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Cat No. gb2AF488

Publications(75)

Reviews (2)

Specificity

GFP and GFP derivates

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 488

Host

Alpaca

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

• Description: The GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

  • Considerably higher tissue penetration rates

  • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

  • Less than 2 nm epitope-label displacement minimizes linkage error

  • Monovalent VHHs do not cluster their epitopes

  • Validation: structure and function characterized

  • Consistent and reliable performance due to recombinant production

• Applications: IF, IHC, ICC
• Host: Alpaca
• Specificity/Target: CFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet; For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
• Conjugate: Alexa Fluor® 488
• Physical State: Liquid
• Suggested Dilution: IF/ICC/IHC: 1:500- 1:1,000
• Type: Nanobody
• Class: Recombinant
• DOL: 2 fluorophores per Nanobody
• Format: Alpaca single domain antibody, Nanobody or VHH; monovalent
• RRID: AB_2827573
• Affinity (KD): Dissociation constant K_D of 1 pM
• Excitation/ Emission: Excitation max: 490 nm, Emission max: 525 nm
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
• Size: 10 μL; 50 μL
• Note: This product is for research use only, not for diagnostic or therapeutic use

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Publications

Application	Title
IF	Nat Biotechnol; Three-dimensional structured illumination microscopy with enhanced axial resolution; Authors - Xuesong Li
IF	Nat Cell Biol; CDK1-cyclin-B1-induced kindlin degradation drives focal adhesion disassembly at mitotic entry.; Authors - Nan-Peng Chen
IF	Mol Cell; Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization.; Authors - Marion E Pesenti
	Nucleic Acids Res; COSA-1 mediated pro-crossover complex formation promotes meiotic crossing over in C. elegans; Authors - Yuejun Yang
IF	Nat Commun; Actin nano-architecture of phagocytic podosomes; Authors - J. Cody Herron
IF	J Exp Med; Bhlhe40 function in activated B and TFH cells restrains the GC reaction and prevents lymphomagenesis.; Authors - René Rauschmeier

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (09-19-2023)

Very good signal enhancement.

• Applications: Western Blot,Immunohistochemistry
• Cell Tissue Type: HCT116

FH

Rocco (Verified Customer) (03-11-2022)

The alpaca GFP booster was very useful to show cellular location of PAR2-mugfp in the mouse colon. It works well at the concentration of 1:400 in a PFA fixed and Sucrose cryoprotected 10um colonic section. A must buy!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Mouse colonic tissue