ChromoTek GFP-Booster Alexa Fluor® 488

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Specificity

GFP and GFP derivates

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 488

Host

Alpaca

Cat no : gb2AF488

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

DescriptionThe GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, IHC, ICC
HostAlpaca
Specificity/TargetCFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
ConjugateAlexa Fluor® 488
Physical StateLiquid
Suggested DilutionIF/ICC/IHC: 1:500- 1:1,000
Type Nanobody
ClassRecombinant
DOL2 fluorophores per Nanobody
RRIDAB_2827573
Affinity (KD) Dissociation constant KD of 1 pM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Stable for 6 month. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Nat Biotechnol

Three-dimensional structured illumination microscopy with enhanced axial resolution

Authors - Xuesong Li
IF

Nat Cell Biol

CDK1-cyclin-B1-induced kindlin degradation drives focal adhesion disassembly at mitotic entry.

Authors - Nan-Peng Chen
IF

Mol Cell

Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization.

Authors - Marion E Pesenti
IF

Nat Commun

Actin nano-architecture of phagocytic podosomes

Authors - J. Cody Herron
IF

J Exp Med

Bhlhe40 function in activated B and TFH cells restrains the GC reaction and prevents lymphomagenesis.

Authors - René Rauschmeier
IF

EMBO J

Embryo-uterine interaction coordinates mouse embryogenesis during implantation

Authors - Vladyslav Bondarenko

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Andrea (Verified Customer) (09-19-2023)

Very good signal enhancement.

  • Applications: Western Blot,Immunohistochemistry
  • Cell Tissue Type: HCT116
FH

Rocco (Verified Customer) (03-11-2022)

The alpaca GFP booster was very useful to show cellular location of PAR2-mugfp in the mouse colon. It works well at the concentration of 1:400 in a PFA fixed and Sucrose cryoprotected 10um colonic section. A must buy!

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Mouse colonic tissue