Cat no : gb2AF488
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
|Applications||IF, IHC, ICC|
|Recommended Dilution||IF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000|
|Affinity||Dissociation constant KD of 1 pM|
|Specificity/Target||AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP|
|Conjugate||Alexa Fluor® 488|
|Storage Buffer||10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide|
|Storage Condition||Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.|
Methods Mol Biol
Correlative microscopy of individual cells: sequential application of microscopic systems with increasing resolution to study the nuclear landscape.
Site-specific recruitment of epigenetic factors with a modular CRISPR/Cas system.
Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair.
Mol Biol Cell
The Borealin dimerization domain interacts with Sgo1 to drive Aurora B-mediated spindle assembly.
Biochim Biophys Acta Mol Cell Res
ErbB3 interacts with Hrs and is sorted to lysosomes for degradation.
Fluorescent TAP as a Platform for Virus-Induced Degradation of the Antigenic Peptide Transporter.