ChromoTek GFP-Booster Alexa Fluor® 488

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Ready to use reagent

Specificity

CFP, eGFP, AcGFP, eYFP

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 488

Cat no : gb2AF488

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

ApplicationsIF, IHC, ICC
Recommended DilutionIF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000
Clonality Monoclonal
Host Alpaca
AffinityDissociation constant KD of 1 pM
Specificity/TargetAcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP
ConjugateAlexa Fluor® 488
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.

Publications

ApplicationTitle
IF

Methods Mol Biol

Correlative microscopy of individual cells: sequential application of microscopic systems with increasing resolution to study the nuclear landscape.

Authors - Barbara Hübner
IF

Nucleus

Site-specific recruitment of epigenetic factors with a modular CRISPR/Cas system.

Authors - Tobias Anton
IF

Nucleus

Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair.

Authors - Vadim O Chagin
IF

Mol Biol Cell

The Borealin dimerization domain interacts with Sgo1 to drive Aurora B-mediated spindle assembly.

Authors - Mary Kate Bonner
IF

Biochim Biophys Acta Mol Cell Res

ErbB3 interacts with Hrs and is sorted to lysosomes for degradation.

Authors - Anne Marthe Fosdahl
IF

Cells

Fluorescent TAP as a Platform for Virus-Induced Degradation of the Antigenic Peptide Transporter.

Authors - Magda Wąchalska