ChromoTek GFP-Booster Alexa Fluor® 488

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Specificity

CFP, eGFP, AcGFP, eYFP

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 488

Publications

16

Cat no : gb2AF488

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

---

Validation Data Gallery

• 

  Confocal image of HeLa cells transiently transfected with Tom70-eGFP and immunostained with GFP-Booster Alexa Fluor 488 (green). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

---

Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

• Applications: IF, IHC, ICC
• Recommended Dilution: IF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP
• Conjugate: 0.5 – 1 mg/mL (conjugates)
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.

Publications

Application	Title
IF	Methods Mol Biol; Correlative microscopy of individual cells: sequential application of microscopic systems with increasing resolution to study the nuclear landscape.; Authors - Barbara Hübner
IF	Nucleus; Site-specific recruitment of epigenetic factors with a modular CRISPR/Cas system.; Authors - Tobias Anton
IF	Nucleus; Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair.; Authors - Vadim O Chagin
IF	Mol Biol Cell; The Borealin dimerization domain interacts with Sgo1 to drive Aurora B-mediated spindle assembly.; Authors - Mary Kate Bonner
IF	Front Cell Neurosci; Generation and Characterization of Immortalized Mouse Cortical Astrocytes From Wildtype and Connexin43 Knockout Mice.; Authors - Antonio Cibelli
IF	Biochim Biophys Acta Mol Cell Res; ErbB3 interacts with Hrs and is sorted to lysosomes for degradation.; Authors - Anne Marthe Fosdahl