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ChromoTek GFP-Booster Alexa Fluor® 488

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Specificity

CFP, eGFP, AcGFP, eYFP

Applications

IF, IHC, ICC

Conjugate

Alexa Fluor® 488

Cat no : gb2AF488

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

Validation Data Gallery

  • Confocal image of HeLa cells transiently transfected with Tom70-eGFP and immunostained with GFP-Booster Alexa Fluor 488 (green). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Confocal image of HeLa cells transiently transfected with Tom70-eGFP and immunostained with GFP-Booster Alexa Fluor 488 (green). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

ApplicationsIF, IHC, ICC
Recommended DilutionIF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 1 pM
Specificity/TargetAcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP
ConjugateAlexa Fluor® 488
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.

Publications

ApplicationTitle
IF

Methods Mol Biol

Correlative microscopy of individual cells: sequential application of microscopic systems with increasing resolution to study the nuclear landscape.

Authors - Barbara Hübner
View Article
IF

Nucleus

Site-specific recruitment of epigenetic factors with a modular CRISPR/Cas system.

Authors - Tobias Anton
View Article
IF

Nucleus

Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair.

Authors - Vadim O Chagin
View Article
IF

Mol Biol Cell

The Borealin dimerization domain interacts with Sgo1 to drive Aurora B-mediated spindle assembly.

Authors - Mary Kate Bonner
View Article
IF

Front Cell Neurosci

Generation and Characterization of Immortalized Mouse Cortical Astrocytes From Wildtype and Connexin43 Knockout Mice.

Authors - Antonio Cibelli
View Article
IF

Biochim Biophys Acta Mol Cell Res

ErbB3 interacts with Hrs and is sorted to lysosomes for degradation.

Authors - Anne Marthe Fosdahl
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Rocco (Verified Customer) (03-11-2022)

The alpaca GFP booster was very useful to show cellular location of PAR2-mugfp in the mouse colon. It works well at the concentration of 1:400 in a PFA fixed and Sucrose cryoprotected 10um colonic section. A must buy!

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Mouse colonic tissue