Cat no : gb2AF488
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
|Applications||IF, IHC, ICC|
|Recommended Dilution||IF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000|
|Affinity||Dissociation constant KD of 1 pM|
|Specificity/Target||AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP|
|Conjugate||0.5 – 1 mg/mL (conjugates)|
|Storage Buffer||10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide|
|Storage Condition||Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.|
Methods Mol Biol
Correlative microscopy of individual cells: sequential application of microscopic systems with increasing resolution to study the nuclear landscape.
Site-specific recruitment of epigenetic factors with a modular CRISPR/Cas system.
Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair.
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The Borealin dimerization domain interacts with Sgo1 to drive Aurora B-mediated spindle assembly.
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Generation and Characterization of Immortalized Mouse Cortical Astrocytes From Wildtype and Connexin43 Knockout Mice.
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