Cat no : gb2AF488
anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.
|Applications||IF, IHC, ICC|
|Recommended Dilution||IF/ICC/IHC: 1:500- 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:100 to 1:4,000|
|Affinity||Dissociation constant KD of 1 pM|
|Specificity/Target||AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP|
|Conjugate||Alexa Fluor® 488|
|Storage Buffer||10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% Sodium azide|
|Storage Condition||Shipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.|
|SDS GFP-Booster Alexa Fluor 488 (PDF)|
|Protocol GFP-Booster Alexa Fluor 488 (PDF)|
|Fluorescent protein specificity table Nano-Booster|
|Chromotek nanobodies brochure (PDF)|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Rocco (Verified Customer) (03-11-2022)
The alpaca GFP booster was very useful to show cellular location of PAR2-mugfp in the mouse colon. It works well at the concentration of 1:400 in a PFA fixed and Sucrose cryoprotected 10um colonic section. A must buy!