ChromoTek GFP-Booster ATTO594

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Ready to use reagent

Specificity

CFP, eGFP, AcGFP, eYFP

Applications

IF, IHC, ICC

Conjugate

ATTO594

Publications

16

Cat no : gba594

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Enhancement of GFP signal with GFP-Booster Atto488: Comparison of signal intensity of a cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.

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Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

• Applications: IF, IHC, ICC
• Recommended Dilution: F/ICC/IHC: 1:200; Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1:50 to 1:1,600
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: AcGFP, Citrine, CFP, eGFP, eYFP, GFP S65T, pHluorin, sfGFP, mClover (Clover A206K), TagGFP, tagGFP2, Venus, wtGFP, YFP
• Conjugate: ATTO594
• Storage Buffer: 1x PBS, 0.09% sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 6 month. Do not freeze. Protect from light.

Publications

Application	Title
IF	PLoS Genet; GOLPH3 is essential for contractile ring formation and Rab11 localization to the cleavage site during cytokinesis in Drosophila melanogaster.; Authors - Stefano Sechi
IF	Cell Stem Cell; Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling.; Authors - Mayank Verma
IF	Int J Mol Sci; Visualization of Bacterial Protein Complexes Labeled with Fluorescent Proteins and Nanobody Binders for STED Microscopy; Authors - Kimberly Cramer
IF	Methods Cell Biol; Subdiffraction resolution microscopy methods for analyzing centrosomes organization.; Authors - Vito Mennella
IF	Mol Microbiol; Growth-driven displacement of protein aggregates along the cell length ensures partitioning to both daughter cells in Caulobacter crescentus.; Authors - Frederic D Schramm
IF	Toxins (Basel); Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells.; Authors - Maximilian Fellermann