Validation Data Gallery
Product Information
The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.
| Description | The GFP-Trap Magnetic Agarose Kit contains GFP-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of GFP-fusion proteins and their interacting factors.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains on the SDS-PAGE • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 15-20 μg of recombinant GFP per 25 μL bead slurry |
| Conjugate | Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 1 pM |
| Compatibility with mass spectrometry | The GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631358 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| GFP-Trap® Magnetic Agarose | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
Documentation
| SDS |
|---|
| SDS GFP-Trap Magnetic Agarose Kit |
| Datasheet |
|---|
| Protocol GFP-Trap_Magnetic Agarose Kit (PDF) |
| GFP-Trap Specificity |
|---|
| Fluorescent protein specificity table |
| Trouble shooting |
|---|
| Troubleshooting guide immunoprecipitation (IP) |
Publications
| Application | Title |
|---|---|
Nature MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function. | |
Cell Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex. | |
Nat Med Subcellular localization of coagulation factor II receptor-like 1 in neurons governs angiogenesis. | |
Nat Cell Biol The Machado-Joseph disease deubiquitylase ATX-3 couples longevity and proteostasis. | |
Nat Commun SepN is a septal junction component required for gated cell–cell communication in the filamentous cyanobacterium Nostoc | |
Mol Cell Nucleo-cytosolic Shuttling of ARGONAUTE1 Prompts a Revised Model of the Plant MicroRNA Pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Mona (Verified Customer) (09-02-2022) | I used the kit to co-immunoprecipitate a GFP tagged protein and it worked well for me. The efficiency was 100%. I would highly recommend it.
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