ChromoTek iST GFP-Trap Kit for AP-MS sample preparation of GFP-fusion proteins

Kit for IP/ Co-IP of GFP-fusion proteins & sample preparation for mass spectrometry (MS)


GFP and GFP derivates







Cat no : gtak-iST


GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

Product Information

Kit for IP/ Co-IP of GFP-fusion proteins & sample preparation for mass spectrometry (MS)

DescriptionThe iST GFP-Trap® Kit enables researchers to process GFP-fusion proteins and their interacting partners for Mass Spectrometry analysis by including the ChromoTek GFP-Trap for immunoprecipitation/Co-IP of GFP-fusion proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls:

• Reliable dentification of GFP-fusion proteins & interacting partners by MS

• Identification of even low abundant/ low expressed GFP-fusion proteins

• Superior processing of IP samples into clean peptide mixtures

• Intuitive protocol for reproducible results

ApplicationsIP, MS
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity25-30 µg of recombinant GFP per 25 µL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Type Nanobody
Affinity (KD) Dissociation constant KD of 1 pM
Compatibility with mass spectrometryThe GFP-Trap® is optimized for on-bead digestion.
Storage Buffer20% ethanol
Storage ConditionKit is shipped at ambient temperature. Upon receipt store GFP-Trap at 4°C and Digest at -20°C

Kit components

GFP-Trap Agarose8 reactions plus 2 controls
DigestEnzyme Trypsin-mix to digest proteins.
Resuspend bufferProtease reconstitution buffer for enzymes.
Lyse bufferDenature, reduce and alkylate proteins.
Stop solutionStop the enzymatic activity.
Wash 1 bufferClean up peptides from hydrophobic contaminants.
Wash 2 bufferClean up peptides from hydrophilic contaminants.
Elution bufferElute the peptides from the cartridge.
LC-Load loadLoad peptides on reversed-phase LC-MS column.
CartridgesCartridge for 1 to 100 µg protein starting material.
Waste tubesTube for collecting waste after washing steps.
Collection tubesTube for collecting peptides after elution.
AdaptersEnables placing a cartridge into a tube.
CapsCap to optionally close the cartridge’s bottom.



J Biol Chem

Phagocytosis mediated by the human granulocyte receptor CEACAM3 is limited by the receptor-type protein tyrosine phosphatase PTPRJ.

Authors - Griseldis Goob

PLoS One

Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking.

Authors - Dieter Waschbüsch


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Caitlin (Verified Customer) (06-23-2022)

It worked well!

  • Applications: Immunoprecipitation
  • Cell Tissue Type: Human IPSC astrocytes