ChromoTek iST GFP-Trap Kit for AP-MS sample preparation of GFP-fusion proteins

Kit for IP/ Co-IP of GFP-fusion proteins & sample preparation for mass spectrometry (MS)

Specificity

GFP, eGFP, CFP, YFP, BFP

Applications

IP, MS

Conjugate

Agarose

Cat no : gtak-iST

Synonyms

GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

Kit for IP/ Co-IP of GFP-fusion proteins & sample preparation for mass spectrometry (MS)

ApplicationsIP, MS
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 1 pM
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
Coupled VHHRecombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind more than 12 µg of recombinant GFP
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
GFP-Trap Agarose 8 reactions plus 2 controls
Digest Enzyme Trypsin-mix to digest proteins.
Resuspend buffer Protease reconstitution buffer for enzymes.
Lyse buffer Denature, reduce and alkylate proteins.
Stop solution Stop the enzymatic activity.
Wash 1 buffer Clean up peptides from hydrophobic contaminants.
Wash 2 buffer Clean up peptides from hydrophilic contaminants.
Elution buffer Elute the peptides from the cartridge.
LC-Load load Load peptides on reversed-phase LC-MS column.
Cartridges Cartridge for 1 to 100 µg protein starting material.
Waste tubes Tube for collecting waste after washing steps.
Collection tubes Tube for collecting peptides after elution.
Adapters Enables placing a cartridge into a tube.
Caps Cap to optionally close the cartridge’s bottom.

Publications

ApplicationTitle
IP

PLoS One

Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking.

Authors - Dieter Waschbüsch