ChromoTek iST GFP-Trap Kit for AP-MS sample preparation of GFP-fusion proteins

Kit for IP/ Co-IP of GFP-fusion proteins & sample preparation for mass spectrometry (MS)

Specificity

GFP, eGFP, CFP, YFP, BFP

Applications

IP, MS

Conjugate

Agarose

Publications

1

Cat no : gtak-iST

Synonyms

GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Make the most of your time & gain about 40 hours with the iST_GFP-Trap Kit.

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  Schematic workflow of iST_GFP-Tap_kit.

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  Preomics Technology Priciple.

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  iST-GFP-Trap Kit for immunoprecipitation and sample preparation for mass spectrometry.

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Product Information

Kit for IP/ Co-IP of GFP-fusion proteins & sample preparation for mass spectrometry (MS)

• Applications: IP, MS
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
• Coupled VHH: Recombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4 % agarose beads)
• Binding capacity: 10 µL slurry bind more than 12 µg of recombinant GFP
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

Component	Description
GFP-Trap Agarose	8 reactions plus 2 controls
Digest	Enzyme Trypsin-mix to digest proteins.
Resuspend buffer	Protease reconstitution buffer for enzymes.
Lyse buffer	Denature, reduce and alkylate proteins.
Stop solution	Stop the enzymatic activity.
Wash 1 buffer	Clean up peptides from hydrophobic contaminants.
Wash 2 buffer	Clean up peptides from hydrophilic contaminants.
Elution buffer	Elute the peptides from the cartridge.
LC-Load load	Load peptides on reversed-phase LC-MS column.
Cartridges	Cartridge for 1 to 100 µg protein starting material.
Waste tubes	Tube for collecting waste after washing steps.
Collection tubes	Tube for collecting peptides after elution.
Adapters	Enables placing a cartridge into a tube.
Caps	Cap to optionally close the cartridge’s bottom.

Publications

Application	Title
IP	PLoS One; Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking.; Authors - Dieter Waschbüsch