ChromoTek GFP-Trap® Agarose

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.

Ready to use reagent

Specificity

GFP, eGFP, CFP, YFP, BFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : gta

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality Monoclonal
Host Alpaca
AffinityDissociation constant KD of 1 pM
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
Coupled VHHRecombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Binding capacity10 µL slurry bind about 12 µg of recombinant GFP
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

PLoS Genet

Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.

Authors - Emily Bowie
IP

Mol Biol Cell

PRMT7 Methylates Eukaryotic Translation Initiation Factor 2α and Regulates its Role in Stress Granule Formation.

Authors - Nasim Haghandish
CoIP

Nat Commun

Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency.

Authors - J Mathieu
IP

Life Sci Alliance

BZLF1 interacts with chromatin remodelers promoting escape from latent infections with EBV.

Authors - Marisa Schaeffner
IP

Nat Commun

The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα.

Authors - Anton Iershov
CoIP

Cell Rep

The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact Formation.

Authors - Dongfang Li