Cat no : gta
GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
|Description||Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to beads.
• Fast, reliable & efficient one-step immunoprecipitation
• No heavy & light antibody chains
• Stable under harsh washing conditions
• Suitable for downstream mass spec analysis
• Works in samples from: mammals, plants, bacteria, yeast, insects etc.
|Applications||IP, CoIP, ChIP, RIP|
|Specificity/Target||AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP |
For the complete list, please click here: Fluorescent protein specificity table
|Binding capacity||25-30 µg of recombinant GFP per 25 µL bead slurry|
|Conjugate||Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)|
|Elution buffer||SDS sample buffer|
0.2 M glycine pH 2.5
|Wash buffer compatibility||1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100|
|Affinity (KD)||Dissociation constant KD of 1 pM|
|Compatibility with mass spectrometry||The GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry|
|Storage Buffer||20% ethanol|
|Storage Condition||Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!|
|SDS GFP-Trap Agarose|
|Protocol GFP-Trap Agarose (PDF)|
|How to plan an Immunoprecipitation using the GFP-Trap|
|Split Fluorescent Protein Technology (PDF)|
|GFP-Trap as affinity tag|
|Unique Stability of the ChromoTek GFP-Trap:GFP Complex|
|Fluorescent protein specificity table|
|Troubleshooting guide immunoprecipitation (IP)|
|GFP-Trap product brochure|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Rich (Verified Customer) (09-12-2022)
works well. A little on the pricey side but worth it.
Aditi (Verified Customer) (08-17-2022)
The product works very well even with non-conventional model systems. We used it for co-immunoprecipitation and mass spectrometry analysis. We used custom buffers and the product worked efficiently. The whole protocol is also quicker than regular IP protocols. Highly recommend!
Benedikt (Verified Customer) (08-17-2022)
We did a pull-down of a ~131 GFP-fusionprotein using the recommended buffers and incubation times (1h) and immediately pulled our protein-of-interest. Some non-bound protein was still visible, although this might be due to high amount of input lysate (500 µg).
Peter (Verified Customer) (07-04-2022)
Our lab has used the GFP-Trap Agarose beads on many occasions to successfully co-immunoprecipitate GFP fusion proteins for Mass Spectrometry and for RNA sequencing (RIP-Seq). We even managed to pulldown enough of one protein to detect it after the western blot on the PVDF membrane with amidoblack staining.
Cristina (Verified Customer) (06-10-2022)
we have been using this product for years and it's one of the fav in the lab
George (Verified Customer) (06-10-2022)
Used this for GFP immunoprecipitations from HeLa cells. Product worked as described and is much quicker and more effective than conventional immunoprecipitations. Would highly recommend.
Cristina (Verified Customer) (03-11-2022)
Great product. It is a daily must have in our lab.