ChromoTek GFP-Trap® Magnetic Particles M-270, Kit for Immunoprecipitation

Validation Data Gallery

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  Immunoprecipitation (IP) of GFP with GFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound.

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  Immunoprecipitation (IP) of GFP with GFP-Trap Magnetic Particles M-270: Coomassie and Western blot (I: Input, FT: Flow-Through, B: Bound).

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  Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.

GFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Magnetic Particles M-270.

Cat No. gtdk

Publications(9)

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic Particles M-270

Synonyms

GFP Trap Magnetic Particles M-270, GFP-Trap Magnetic Particles M-270, GFP-Trap Magnetic Particles M-270 kit, GFP-Trap® Magnetic Particles M-270 kit, gtdk, gtdk-20, gfp, GFP tag, GFP-tag, GFP-Trap® Magnetic Particles M-270, gtdk-20

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Product Information

GFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of GFP-tagged proteins. GFP-Trap® Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a GFP VHH/ Nanobody coupled to Magnetic Particles M-270.

• Description: The GFP-Trap Magnetic Particles M-270 Kit contains GFP-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of GFP-fusion proteins and their interacting factors.

  • IP and Co-IP of GFP-tagged proteins without size limitation

  • Magnetic separation with easy and efficient washing of GFP-Trap Magnetic Particles M-270

  • Automation and high throughput applications

  • No heavy & light antibody chains, short incubation (5-30 min)

  • Extraordinary binding, also under harsh conditions

  • Very high affinity (KD=1 pM) to bind even low abundant proteins

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 2-2.8 μg of recombinant GFP per 25 μL bead slurry
• Conjugate: Magnetic Particles M-270, size: 2.8 µm; high throughput-compatible
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 10 mM DTT, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 0.2 % SDS, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 1 pM
• Compatibility with mass spectrometry: The GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2827592
• Storage Buffer: PBS with 0.09% sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

• Component: Description
• Lysis buffer: Optimized for cytoplasmatic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate
• Elution buffer: For acidic elution
• GFP-Trap® Magnetic Particles M-270: 20 reactions (500 µL)

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Publications

Application	Title
IP	Nat Commun; Remodeling the cellular stress response for enhanced genetic code expansion in mammalian cells; Authors - Mikhail E Sushkin
IP	EMBO Rep; Foxd3 controls heterochromatin-mediated repression of repeat elements and 2-cell state transcription.; Authors - Deepika Puri
IF	PLoS Biol; Genome-wide functional analysis reveals key roles for kinesins in the mammalian and mosquito stages of the malaria parasite life cycle; Authors - Mohammad Zeeshan
IP	Exp Cell Res; YBX2 modulates mRNA stability via interaction with YTHDF2 in endometrial cancer cells; Authors - Ying Cai
IP	Cell Rep; Dual agonistic and antagonistic roles of ZC3H18 provide for co-activation of distinct nuclear RNA decay pathways; Authors - Patrik Polák
IP	Cell Mol Life Sci; Inhibition of autocrine HGF maturation overcomes cetuximab resistance in colorectal cancer; Authors - Vivian Truong Jones