ChromoTek GFP-Trap® Magnetic Agarose, kit

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

Specificity

GFP, eGFP, CFP, YFP, BFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Cat no : gtmak

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality VHH
HostAlpaca
AffinityDissociation constant KD of 1 pM
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
Coupled VHHRecombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment
ConjugateMagnetic Agarose
Beads~ 40 µm
Binding capacity10 µL slurry bind more than 8 µg of recombinant GFP
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
GFP-Trap® Magnetic Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
IP

Oncotarget

Septin cooperation with tubulin polyglutamylation contributes to cancer cell adaptation to taxanes.

Authors - Laurence Froidevaux-Klipfel
IP

Biochim Biophys Acta

Interaction with epsin 1 regulates the constitutive clathrin-dependent internalization of ErbB3.

Authors - Monika Szymanska
IP

Cell Rep

OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation.

Authors - Mingyi Yang
IP

FEBS Lett

Rab7a and Rab27b control secretion of endothelial microRNA through extracellular vesicles.

Authors - Nicolas Jaé
IP

Sci Rep

Peroxisomes are platforms for cytomegalovirus' evasion from the cellular immune response.

Authors - Ana Cristina Magalhães
IP

J Cell Mol Med

A combination of two antibodies recognizing non-overlapping epitopes of HER2 induces kinase activity-dependent internalization of HER2.

Authors - Monika Szymanska