Cat no : 3h9
anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins
|Recommended Dilution||IF: 1:10,000; Immunoblotting: 1:1,000 to 1:20,000; Optimal working concentration is application-dependent and should be determined by testing|
|Affinity||Dissociation constant KD of 6 nM|
|Conjugate||0.5 – 1 mg/mL (conjugates)|
|Storage Buffer||Buffered aqueous solution (PBS)|
|Storage Condition||Shipped at ambient temperature. Upon receipt store at +4°C / 40°F.Stable for 6 months. Do not freeze. Protect from light.|
Methods Mol Biol
Fluorescent protein specific Nanotraps to study protein-protein interactions and histone-tail peptide binding.
Mol Plant Pathol
A barley SKP1-like protein controls abundance of the susceptibility factor RACB and influences the interaction of barley with the barley powdery mildew fungus.
Exocyst-Dependent Membrane Addition Is Required for Anaphase Cell Elongation and Cytokinesis in Drosophila.
The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport.
Scribbled Optimizes BMP Signaling through Its Receptor Internalization to the Rab5 Endosome and Promote Robust Epithelial Morphogenesis.
Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis.