ChromoTek GFP Monoclonal antibody (3H9)

Rat monoclonal antibody [3H9] to Green Fluorescent Proteins

Host/IsoType

Rat/IgG2a

Specificity

GFP

Applications

WB

Conjugate

Unconjugated

Clone No.

3H9

Publications

318

Reviews (1)

Cat no : 3h9

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Dilution series of EGFP in HEK 293T cell lysate Primary antibody: [3H9] 1:1,000 Secondary antibody: goat anti-rat Alexa Fluor® 488 1:1,000

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Product Information

Rat monoclonal antibody [3H9] to Green Fluorescent Proteins

• Description: GFP rat monoclonal antibody [3H9] for WB
• Applications: WB
• Specificity/Target: The antibody recognizes green fluorescent proteins derived from Aequorea victoria GFP, such as eGFP, wtGFP, YFP, or CFP.; For the complete list, please click here: Fluorescent protein specificity table antibodies
• Recommended Dilution: Western Blot: 1:1,000; Note: GFP antibody [3H9] does not work in immunofluorescence.
• Host/IsoType: Rat/IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Green Fluorescent Protein (GFP)
• UniProtKB: P42212
• Clone No.: 3H9
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A or G purified
• RRID: AB_10773374
• Storage Buffer: PBS, 50% glycerol, preservative: 0.09% sodium azide.
• Storage Condition: Shipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment.

Publications

Application	Title
WB	Nature; A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.; Authors - Evelyn Fessler
WB	Nature; TMK-based cell-surface auxin signalling activates cell-wall acidification.; Authors - Wenwei Lin
IP	Cell; A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling.; Authors - Noémie Alphonse
IF	Cell; The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover.; Authors - Riga Tawo
WB	Cell; Exome capture reveals ZNF423 and CEP164 mutations, linking renal ciliopathies to DNA damage response signaling.; Authors - Moumita Chaki
IF	Nat Methods; Quantitative mapping and minimization of super-resolution optical imaging artifacts.; Authors - Siân Culley

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (09-19-2023)

The antibody works well, strong signal, clear background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250