ChromoTek GFP Monoclonal antibody (3H9)

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

Specificity

Spot-Tag

Applications

IF, WB

Conjugate

0.5 – 1 mg/mL (conjugates)

Cat no : 3h9

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Label



Product Information

anti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins

ApplicationsIF, WB
Recommended DilutionIF: 1:10,000; Immunoblotting: 1:1,000 to 1:20,000; Optimal working concentration is application-dependent and should be determined by testing
Clonality Monoclonal
HostAlpaca
AffinityDissociation constant KD of 6 nM
Specificity/TargetSpot-Tag (PDRVRAVSHWSS)
GeneID (NCBI)0
Conjugate0.5 – 1 mg/mL (conjugates)
Storage BufferBuffered aqueous solution (PBS)
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C / 40°F.Stable for 6 months. Do not freeze. Protect from light.

Publications

ApplicationTitle
WB

Methods Mol Biol

Fluorescent protein specific Nanotraps to study protein-protein interactions and histone-tail peptide binding.

Authors - Garwin Pichler
WB

Mol Plant Pathol

A barley SKP1-like protein controls abundance of the susceptibility factor RACB and influences the interaction of barley with the barley powdery mildew fungus.

Authors - Tina Reiner
WB

PLoS Genet

Exocyst-Dependent Membrane Addition Is Required for Anaphase Cell Elongation and Cytokinesis in Drosophila.

Authors - Maria Grazia Giansanti
WB

Oncotarget

The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport.

Authors - Maria L Torgersen
IHC

PLoS Genet

Scribbled Optimizes BMP Signaling through Its Receptor Internalization to the Rab5 Endosome and Promote Robust Epithelial Morphogenesis.

Authors - Jinghua Gui
WB,IP

PLoS Genet

Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis.

Authors - Marion Miller