GFRA1 Polyclonal antibody

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  WB analysis using 16324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16324-1-AP (GFRA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GFRA1 Polyclonal Antibody for WB, ELISA

Cat No. 16324-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GDNF family receptor alpha 1, GDNF family receptor alpha-1, GDNF receptor alpha 1, GDNF receptor alpha-1, GDNFR

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Tested Applications

• Positive WB detected in: SK-N-SH cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16324-1-AP targets GFRA1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9407

  Product name: Recombinant human GFRA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 105-460 aa of BC014962

  Sequence: SMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASSHITTKSMAAPPSCGLSPLLVLVVTALSTLLSLTETS

• Full Name: GDNF family receptor alpha 1
• Calculated Molecular Weight: 460 aa, 51 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC014962
• Gene Symbol: GFRA1
• Gene ID (NCBI): 2674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56159
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFRA1, also named as GDNFRA, RETL1 and TRNR1, is a receptor for GDNF. It mediates the GDNF-induced autophosphorylation and activation of the RET receptor. GFRA1 has two isoforms with MW 45-50 kDa and 50-55 kDa.