Tested Applications
| Positive WB detected in | HepG2 cells, mouse brain tissue, L02 cells, U-87 MG cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21973-1-AP targets GFRA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16893 Product name: Recombinant human GFRA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 270-464 aa of BC041688 Sequence: DFHANCRASYQTVTSCPADNYQACLGSYAGMIGFDMTPNYVDSSPTGIVVSPWCSCRGSGNMEEECEKFLRDFTENPCLRNAIQAFGNGTDVNVSPKGPSFQATQAPRVEKTPSLPDDLSDSTSLGTSVITTCTSVQEQGLKANNSKELSMCFTELTTNIIPGSNKVIKPNSGPSRARPSAALTVLSVLMLKLAL Predict reactive species |
| Full Name | GDNF family receptor alpha 2 |
| Calculated Molecular Weight | 464 aa, 52 kDa |
| Observed Molecular Weight | 34-40 kDa, 50-55 kDa |
| GenBank Accession Number | BC041688 |
| Gene Symbol | GFRA2 |
| Gene ID (NCBI) | 2675 |
| RRID | AB_11124728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00451 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GFRA2, also named as GDNFRB, RETL2 and TRNR2, belongs to the GDNFR family. It is a receptor for neurturin. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It is a potent survival factor for central dopaminergic neurons, motor neurons, and several other populations of neurons in the central and peripheral nervous systems. The GDNF signal is mediated by a complex of the receptor tyrosine kinase RET and a glycosylphosphatidylinositol (GPI)-linked protein, GDNFRA. GFRA2 has three isoforms with MW 34-36 kDa, 39-41 kDa and 50-55 kDa. This antibody recognizes all the three isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GFRA2 antibody 21973-1-AP | Download protocol |
| WB protocol for GFRA2 antibody 21973-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GFRA2 Polyclonal antibody (Cat# 21973-1-AP) has 4 citations published in Cancer Letters, Journal of Pathology, Scientific Reports, and Biotechnology and Applied Biochemistry. The research fields span pancreatic cancer (chemoresistance and PTEN regulation), neuroblastoma microenvironment (spatial transcriptomics), hypoxia adaptation in the peripheral auditory system, and neuroprotection via astroglial Nrf2 regulation. Applications cited include IHC and WB in human and rat samples.
| Species | Application | Title |
|---|---|---|
Cancer Lett GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer. | ||
J Pathol Spatial transcriptomics exploration of the primary neuroblastoma microenvironment in archived FFPE samples unveils novel paracrine interactions. | ||
Sci Rep Hypoxia adaptation mechanism in rats' peripheral auditory system in high altitude migration: a time series transcriptome analysis | ||
