GFRA2 Polyclonal antibody

GFRA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21973-1-AP

Synonyms

GDNF family receptor alpha 2, GDNF receptor alpha 2, GDNF receptor beta, GDNFR alpha 2, GDNFR beta, GDNFRB, GFR alpha 2, GFRA2, Neurturin receptor alpha, NRTNR ALPHA, NTNR alpha, NTNRA, RET ligand 2, RETL2, TRNR2

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Validation Data Gallery

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  WB analysis of mouse brain using 21973-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21973-1-AP (GFRA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21973-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21973-1-AP (GFRA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21973-1-AP (GFRA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 21973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21973-1-AP (GFRA2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, multi-cells/tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 21973-1-AP is GFRA2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GFRA2 fusion protein Ag16893
• Full Name: GDNF family receptor alpha 2
• Calculated Molecular Weight: 464 aa, 52 kDa
• Observed Molecular Weight: 34-40 kDa, 50-55 kDa
• GenBank Accession Number: BC041688
• Gene Symbol: GFRA2
• Gene ID (NCBI): 2675
• RRID: AB_11124728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFRA2, also named as GDNFRB, RETL2 and TRNR2, belongs to the GDNFR family. It is a receptor for neurturin. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It is a potent survival factor for central dopaminergic neurons, motor neurons, and several other populations of neurons in the central and peripheral nervous systems. The GDNF signal is mediated by a complex of the receptor tyrosine kinase RET and a glycosylphosphatidylinositol (GPI)-linked protein, GDNFRA. GFRA2 has three isoforms with MW 34-36 kDa, 39-41 kDa and 50-55 kDa. This antibody recognizes all the three isoforms.

Publications

Species	Application	Title
human	IHC	Cancer Lett; GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer.; Authors - Jiangning Gu
rat	WB	Biotechnol Appl Biochem; Ellagic acid supports neuron by regulating astroglia Nrf2.; Authors - Guo-Qing Wang