GFRA2 Polyclonal antibody

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  WB analysis using 21973-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21973-1-AP (GFRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21973-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21973-1-AP (GFRA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21973-1-AP (GFRA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 21973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21973-1-AP (GFRA2 antibody) at dilution of 1:50 (under 40x lens).

GFRA2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21973-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

GDNF family receptor alpha 2, GDNF family receptor alpha-2, GDNF receptor alpha 2, GDNF receptor alpha-2, GDNF receptor beta

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, L02 cells, U-87 MG cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

21973-1-AP targets GFRA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GFRA2 fusion protein Ag16893
• Full Name: GDNF family receptor alpha 2
• Calculated Molecular Weight: 464 aa, 52 kDa
• Observed Molecular Weight: 34-40 kDa, 50-55 kDa
• GenBank Accession Number: BC041688
• Gene Symbol: GFRA2
• Gene ID (NCBI): 2675
• RRID: AB_11124728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00451
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFRA2, also named as GDNFRB, RETL2 and TRNR2, belongs to the GDNFR family. It is a receptor for neurturin. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It is a potent survival factor for central dopaminergic neurons, motor neurons, and several other populations of neurons in the central and peripheral nervous systems. The GDNF signal is mediated by a complex of the receptor tyrosine kinase RET and a glycosylphosphatidylinositol (GPI)-linked protein, GDNFRA. GFRA2 has three isoforms with MW 34-36 kDa, 39-41 kDa and 50-55 kDa. This antibody recognizes all the three isoforms.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Cancer Lett; GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer.; Authors - Jiangning Gu
rat	IHC	Sci Rep; Hypoxia adaptation mechanism in rats' peripheral auditory system in high altitude migration: a time series transcriptome analysis; Authors - Luoying Wang
rat	WB	Biotechnol Appl Biochem; Ellagic acid supports neuron by regulating astroglia Nrf2.; Authors - Guo-Qing Wang