GFRA2 Polyclonal antibody

GFRA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21973-1-AP

Synonyms

GDNF family receptor alpha 2, GDNF receptor alpha 2, GDNF receptor beta, GDNFR alpha 2, GDNFR beta, GDNFRB, GFR alpha 2, GFRA2, Neurturin receptor alpha, NRTNR ALPHA, NTNR alpha, NTNRA, RET ligand 2, RETL2, TRNR2



Tested Applications

Positive WB detected inHepG2 cells, multi-cells/tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21973-1-AP is GFRA2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFRA2 fusion protein Ag16893
Full Name GDNF family receptor alpha 2
Calculated Molecular Weight 464 aa, 52 kDa
Observed Molecular Weight 34-40 kDa, 50-55 kDa
GenBank Accession NumberBC041688
Gene Symbol GFRA2
Gene ID (NCBI) 2675
RRIDAB_11124728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GFRA2, also named as GDNFRB, RETL2 and TRNR2, belongs to the GDNFR family. It is a receptor for neurturin. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It is a potent survival factor for central dopaminergic neurons, motor neurons, and several other populations of neurons in the central and peripheral nervous systems. The GDNF signal is mediated by a complex of the receptor tyrosine kinase RET and a glycosylphosphatidylinositol (GPI)-linked protein, GDNFRA. GFRA2 has three isoforms with MW 34-36 kDa, 39-41 kDa and 50-55 kDa. This antibody recognizes all the three isoforms.

Protocols

Product Specific Protocols
WB protocol for GFRA2 antibody 21973-1-APDownload protocol
IHC protocol for GFRA2 antibody 21973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Lett

GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer.

Authors - Jiangning Gu
ratWB

Biotechnol Appl Biochem

Ellagic acid supports neuron by regulating astroglia Nrf2.

Authors - Guo-Qing Wang