GGA1 Polyclonal antibody

GGA1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 25674-1-AP

Synonyms

GGA1

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Validation Data Gallery

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  WB analysis of HEK-293 using 25674-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25674-1-AP (GGA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25674-1-AP (GGA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 25674-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25674-1-AP (GGA1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 25674-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25674-1-AP (GGA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 25674-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25674-1-AP (GGA1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, rat brain tissue, HeLa cells, SH-SY5Y cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

25674-1-AP targets GGA1 in WB, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GGA1 fusion protein Ag22614
• Full Name: golgi associated, gamma adaptin ear containing, ARF binding protein 1
• Observed Molecular Weight: 70-72 kDa
• GenBank Accession Number: BC044629
• Gene Symbol: GGA1
• Gene ID (NCBI): 26088
• RRID: AB_2880188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Rep; Golgi retention and oncogenic KIT signaling via PLCγ2-PKD2-PI4KIIIβ activation in gastrointestinal stromal tumor cells; Authors - Yuuki Obata ; KD Validated
mouse	WB	Environ Toxicol; Total flavonoids of Rhizoma drynariae improves tendon-bone healing for anterior cruciate ligament reconstruction in mice and promotes the osteogenic differentiation of bone mesenchymal stem cells by the ERR1/2-Gga1-TGF-β/MAPK pathway; Authors - Lei Han

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Cristian (Verified Customer) (04-17-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 25674-1-AP (GGA1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

GGA1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:25674-1-AP)