GGPS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GGPS1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 14944-1-AP

Synonyms

GGPP synthase, GGPPS, GGPPS1, GGPPSase, GGPS1

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Validation Data Gallery

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  WB analysis of mouse brain using 14944-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14944-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14944-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 14944-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14944-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14944-1-AP

  IP result of anti-GGPS1 (IP:14944-1-AP, 4ug; Detection:14944-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human kidney using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14944-1-AP (GGPS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14944-1-AP (GGPS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14944-1-AP (GGPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14944-1-AP (GGPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14944-1-AP (GGPS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14944-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14944-1-AP (GGPS1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human placenta tissue, human testis tissue, mouse bladder tissue, human heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, human liver tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Product Information

14944-1-AP targets GGPS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GGPS1 fusion protein Ag6745
• Full Name: geranylgeranyl diphosphate synthase 1
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC067768
• Gene Symbol: GGPS1
• Gene ID (NCBI): 9453
• RRID: AB_2110990
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GGPS1(Geranylgeranyl pyrophosphate synthase) belongs to the FPP/GGPP synthase family and catalyzes the synthesis of GGPP, an important molecule responsible for the C20-prenylation of protein and for the regulation of a nuclear hormone receptor. GGPP synthase puri¢ed from bovine brain is reported to be a homo-oligomer (150-195 kDa) with a molecular mass of 37.5 kDa for the monomer. This is consistent with the calculated molecular mass (35 kDa) of mouse and human GGPP synthases (PMID:10101267).

Publications

Species	Application	Title
mouse	IF	Dev Cell; Quantitative proteomic profiling identifies global protein network dynamics in murine embryonic heart development; Authors - Whitney Edwards
mouse	WB	FEBS J; Conditional Loss of Geranylgeranyl Diphosphate Synthase Alleviates Acute Obstructive Cholestatic Liver Injury by Regulating Hepatic Bile Acid Metabolism.; Authors - Wen-Jun Jia
	IHC	Am J Pathol; Geranylgeranyl Diphosphate Synthase Modulates Fetal Lung Branching Morphogenesis Possibly through Controlling K-Ras Prenylation.; Authors - Wen-Jun Jia
human	WB,IHC	J Cell Mol Med; Overexpression of geranylgeranyl diphosphate synthase contributes to tumour metastasis and correlates with poor prognosis of lung adenocarcinoma.; Authors - Xiaoxia Wang ; KD Validated
mouse	WB,IF	Cells; Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model.; Authors - Natalie Längst
human	WB	J Hepatocell Carcinoma; miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway; Authors - Kailin Xing