GGT7 Polyclonal antibody

• 

  WB analysis of HEK-293 using 24674-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24674-1-AP (GGT7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 24674-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24674-1-AP (GGT7 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 24674-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24674-1-AP (GGT7 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung using 24674-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24674-1-AP (GGT7 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung using 24674-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 24674-1-AP (GGT7 Antibody) at dilution of 1:50.

GGT7 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24674-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

D20S101, gamma glutamyltransferase 7, Gamma glutamyltranspeptidase 7, GGT 7, GGT4, GGT7, GGTL3, GGTL5

---

Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

24674-1-AP targets GGT7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20234

  Product name: Recombinant human GGT7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 313-662 aa of BC141843

  Sequence: VLDVLGTSGPAAFYAGGNLTLEMVAEAQHAGGVITEEDFSNYSALVEKPVCGVYRGHLVLSPPPPHTGPALISALNILEGFNLTSLVSREQALHWVAETLKIALALASRLGDPVYDSTITESMDDMLSKVEAAYLRGHINDSQAAPAPLLPVYELDGAPTAAQVLIMGPDDFIVAMVSSLNQPFGSGLITPSGILLNSQMLDFSWPNRTANHSAPSLENSVQPGKRPLSFLLPTVVRPAEGLCGTYLALGANGAARGLSGLTQVLLNVLTLNRNLSDSLARGRLHPDLQSNLLQVDSEFTEEEIEFLEARGHHVEKVDVLSWVHGSRRTNNFIIAVKDPRSPDAAGATIL

• Full Name: gamma-glutamyltransferase 7
• Calculated Molecular Weight: 662 aa, 70 kDa
• Observed Molecular Weight: 75-100 kDa
• GenBank Accession Number: BC141843
• Gene Symbol: GGT7
• Gene ID (NCBI): 2686
• RRID: AB_2879667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJ14
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	IF	iScience; Rapid fluorescent vital imaging of olfactory epithelium.; Authors - Hironobu Nishijima
human	IHC	bioRxiv; Perturbations of glutathione and sphingosine metabolites in Port Wine Birthmark patient-derived induced pluripotent stem cells; Authors - Vi Nguyen
human	IHC	Metabolites; Perturbations of Glutathione and Sphingosine Metabolites in Port Wine Birthmark Patient-Derived Induced Pluripotent Stem Cells; Authors - Vi Nguyen
human,mouse	WB,IP	Genome Biol; Systematic interrogation of functional genes underlying cholesterol and lipid homeostasis; Authors - Haihuan Shan ; KO Validated