GH2 Polyclonal antibody

GH2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55240-1-AP

Synonyms

GH V, GH2, GHL, GHV, hGH V

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Validation Data Gallery

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  WB analysis of human placenta using 55240-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55240-1-AP (GH2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 55240-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55240-1-AP (GH2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 55240-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55240-1-AP (GH2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pituitary adenoma using 55240-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 55240-1-AP (GH2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pituitary adenoma using 55240-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 55240-1-AP (GH2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human placenta tissue, human pituitary adenoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55240-1-AP targets GH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GH2
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: NM_002059
• Gene Symbol: GH2
• Gene ID (NCBI): 2689
• RRID: AB_10898172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GH2, also named as GH-V, belongs to the somatotropin/prolactin family. It plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. This antibody is specific to GH2.