GHDC Polyclonal antibody

GHDC Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26123-1-AP

Synonyms

D11LGP1, D11LGP1E, GH3 domain containing, GH3 domain containing protein, GHDC, LGP1

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Validation Data Gallery

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  WB analysis of HEK-293 using 26123-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26123-1-AP (GHDC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 26123-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26123-1-AP (GHDC Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 26123-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26123-1-AP (GHDC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 26123-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26123-1-AP (GHDC Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26123-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26123-1-AP (GHDC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HEK-293 using 26123-1-AP

  Immunofluorescent analysis of HEK-293 cells using 26123-1-AP (GHDC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human brain tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26123-1-AP is GHDC Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GHDC fusion protein Ag23480
• Full Name: GH3 domain containing
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC022784
• Gene Symbol: GHDC
• Gene ID (NCBI): 84514
• RRID: AB_2880391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.