Filter: AllWBIHCIF/ICC

WB analysis of HEK-293 using 26123-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 26123-1-AP (GHDC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 26123-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26123-1-AP (GHDC Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 26123-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26123-1-AP (GHDC Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 26123-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26123-1-AP (GHDC Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 26123-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26123-1-AP (GHDC Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HEK-293 using 26123-1-AP
Immunofluorescent analysis of HEK-293 cells using 26123-1-AP (GHDC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GHDC Polyclonal antibody

Name: GHDC Polyclonal antibody
Brand: Proteintech
SKU: 26123-1-AP

Cat No. 26123-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

D11LGP1, D11LGP1E, GH3 domain containing, GH3 domain containing protein, GHDC, LGP1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	HEK-293 cells
Positive IHC detected in	human brain tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

26123-1-AP targets GHDC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag23480 Product name: Recombinant human GHDC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 140-429 aa of BC022784 Sequence: KAYPEVLAQGRTARVTLTSPWPRPLPWPGNTLGQVGTPGTKDPRALLLDALRSPGLRALEAGTAVELLDVFLGLETDGEELAGAIAAGNPGAPLRERAAELREALEQGPRGLALRLWPKLQVVVTLDAGGQAEAVAALGALWCQGLAFFSPAYAASGGVLGLNLQPEQPHGLYLLPPGAPFIELLPVKEGTQEEAASTLLLAEAQQGKEYELVLTDRASLTRCRLGDVVRVVGAYNQCPVVRFICRLDQTLSVRGEDIGEDLFSEALGRAVGQWAGAKLLDHGCVESSIL Predict reactive species
Full Name	GH3 domain containing
Observed Molecular Weight	57 kDa
GenBank Accession Number	BC022784
Gene Symbol	GHDC
Gene ID (NCBI)	84514
RRID	AB_2880391
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N2G8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for GHDC antibody 26123-1-AP	Download protocol
IHC protocol for GHDC antibody 26123-1-AP	Download protocol
WB protocol for GHDC antibody 26123-1-AP	Download protocol