GHRL Polyclonal antibody

GHRL Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13309-1-AP

Synonyms

Appetite regulating hormone, Ghrelin Obestatin, GHRL, Motilin related peptide, MTLRP, obestatin, Protein M46



Tested Applications

Positive WB detected inhuman stomach tissue
Positive IHC detected inhuman stomach tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

13309-1-AP targets GHRL in WB, IHC,ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GHRL fusion protein Ag4141
Full Name ghrelin/obestatin prepropeptide
Calculated molecular weight 117 aa, 13 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC025791
Gene symbol GHRL
Gene ID (NCBI) 51738
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is predominantly produced in endocrine cells in the gastric mucosa, called X/A-like or ghrelin cells, from where it is secreted into the plasma. In the pituitary gland, ghrelin stimulates GH release and regulates food intake and energy metabolism. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes.

Protocols

Product Specific Protocols
WB protocol for GHRL antibody 13309-1-APDownload protocol
IHC protocol for GHRL antibody 13309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Hepatobiliary Pancreat Dis Int

Ghrelin inhibits IKKβ/NF-κB activation and reduces pro-inflammatory cytokine production in pancreatic acinar AR42J cells treated with cerulein.

Authors - Ren-Jie Chang
ratWB

Effects of Ghrelin miRNA on Inflammation and Calcium Pathway in Pancreatic Acinar Cells of Acute Pancreatitis

Authors -
mouseIHC

Neuroreport

Expression of ghrelin or growth hormone secretagogue receptor in the brain of postpartum stress mice.

Authors - Jing-Wei Xing
humanIHC

Biosci Rep

Differential expression of ghrelin and GHSR via the mTOR pathway during the dynamic carcinogenic process involving oral, potentially malignant disorders.

Authors - Jianing Lou