GIGYF2 Polyclonal antibody

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  WB analysis using 24790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24790-1-AP (GIGYF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24790-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24790-1-AP (GIGYF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 24790-1-AP

  IP result of anti-GIGYF2 (IP:24790-1-AP, 4ug; Detection:24790-1-AP 1:2000) with Jurkat cells lysate 2000ug.

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  IHC staining of rat brain using 24790-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24790-1-AP (GIGYF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24790-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24790-1-AP (GIGYF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24790-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GIGYF2 antibody (24790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

GIGYF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24790-1-AP

Publications(10)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

GRB10-interacting GYF protein 2, GYF2, KIAA0642, PERQ amino acid-rich with GYF domain-containing protein 2, PERQ2

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, NIH/3T3 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

24790-1-AP targets GIGYF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20329

  Product name: Recombinant human GIGYF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 777-1104 aa of BC146775

  Sequence: KRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELEVQRQKELMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPSGTTKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSNADTKNSNMGFWDDAV

• Full Name: GRB10 interacting GYF protein 2
• Calculated Molecular Weight: 1320 aa, 152 kDa
• Observed Molecular Weight: 150-170 kDa
• GenBank Accession Number: BC146775
• Gene Symbol: GIGYF2
• Gene ID (NCBI): 26058
• RRID: AB_2879727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6Y7W6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIGYF2 (GRB10 interacting GYF protein 2) was initially identified through its interaction with GRB10, an adapter protein that binds activated INS-like growth factor (IGF-I) and INS receptors (PMID: 12771153; 19744960). It may act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. The gene of GIGYF2 has been proposed as the Parkinson-disease (PD) gene underlying the PARK11 locus (PMID: 18358451).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB	Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
mouse,human	WB,IF	Redox Biol; Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function; Authors - Fanglin Niu ; KD Validated
human	WB	Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
mouse	IHC	Biomedicines; Comparative Proteomic Analysis of tPVAT during Ang II Infusion.; Authors - Xiuying Liang
human	WB,IF	iScience; The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.; Authors - Limei Zou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (10-08-2024)

works okay, bands are not the sharpest

• Applications: Western Blot

FH

Parisa (Verified Customer) (09-26-2023)

Good qulity, we could see some other non-specific bands close to the predicted GIGYF2 size and we could tell which one is GIGYF2 using the KO cells. But the antibody has a good specificity.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293