GIMAP2 Polyclonal antibody

GIMAP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13445-1-AP

Synonyms

GIMAP2, GTPase IMAP family member 2, GTPase, IMAP family member 2, HIMAP2, IMAP2, Immunity associated protein 2

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Validation Data Gallery

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  WB analysis of human heart using 13445-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13445-1-AP (GIMAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 13445-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13445-1-AP (GIMAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 13445-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13445-1-AP (GIMAP2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 13445-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13445-1-AP (GIMAP2 antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: human heart tissue, human kidney tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13445-1-AP targets GIMAP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GIMAP2 fusion protein Ag4273
• Full Name: GTPase, IMAP family member 2
• Calculated Molecular Weight: 337 aa, 38 kDa
• Observed Molecular Weight: 28-40 kDa
• GenBank Accession Number: BC032345
• Gene Symbol: GIMAP2
• Gene ID (NCBI): 26157
• RRID: AB_2111871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This protein was not eliminated clearly. It belongs to GTPase of the immunity-associated protein family. One of its homologous protein, GIMAP1, runs as a 30kd protein in SDS-PAGE, lower than the calculated molecular weight. This antibody detected one 28kd band in several tissues.