GIMAP7 Polyclonal antibody

• Featured Product
• KD/KO Validated

GIMAP7 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17293-1-AP

Synonyms

Immunity-associated nucleotide 7 protein, IAN-7, IAN7, GTPase IMAP family member 7

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Validation Data Gallery

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  WB analysis of Jurkat using 17293-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17293-1-AP (GIMAP7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17293-1-AP

  IP result of anti-GIMAP7 (IP:17293-1-AP, 4ug; Detection:17293-1-AP 1:800) with Jurkat cells lysate 1080ug.

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  IF Staining of HepG2 using 17293-1-AP

  Immunofluorescent analysis of HepG2 cells, using GIMAP7 antibody 17293-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

17293-1-AP targets GIMAP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GIMAP7 fusion protein Ag11171
• Full Name: GTPase, IMAP family member 7
• Calculated Molecular Weight: 300 aa, 35 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC027613
• Gene Symbol: GIMAP7
• Gene ID (NCBI): 168537
• RRID: AB_2111885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIMAP7, also known as hIAN7 or IAN 7, is a transmembrane protein located in the endoplasmic reticulum and Golgi apparatus. GIMAP7 can promote oxidative stress and apoptosis in ovarian granulosa cells in Polycystic ovary syndrome by inhibiting the SHH signalling pathway(PMID: 36581994). The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.

Publications

Species	Application	Title
human	IHC	Front Oncol; Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.; Authors - Jinguo Zhang
human,rat	WB,IHC	J Ovarian Res; GIMAP7 induces oxidative stress and apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inhibiting sonic hedgehog signalling pathway; Authors - Anran Xu ; KD Validated
human,mouse	WB,IHC	Thorac Cancer; GIMAP7 inhibits epithelial-mesenchymal transition and glycolysis in lung adenocarcinoma cells via regulating the Smo/AMPK signaling pathway; Authors - Liyuan Cui ; KD Validated