Tested Applications
| Positive IHC detected in | human pancreas tissue, rat pancreas tissue, mouse pancreas tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18034-1-AP targets GIP in IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12634 Product name: Recombinant human GIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-153 aa of BC069663 Sequence: EKKEGHFSALPSLPVGSHAKVSSPQPRGPRYAEGTFISDYSIAMDKIHQQDFVNWLLAQKGKKNDWKHNITQREARALELASQANRKEEEAVEPQSSPAKNPSDEDLLRDLLIQELLACLLDQTNLCRLRSR Predict reactive species |
| Full Name | gastric inhibitory polypeptide |
| Calculated Molecular Weight | 153 aa, 17 kDa |
| GenBank Accession Number | BC069663 |
| Gene Symbol | GIP |
| Gene ID (NCBI) | 2695 |
| RRID | AB_2878484 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GIP is an incretin hormone and belongs to the glucagon superfamily. GIP is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption.GIP stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GIP antibody 18034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product is highly positive, rated 5 out of 5 stars. The reviewer used the antibody for immunohistochemistry on human small intestine tissue at a 1:200 dilution and reported that it "worked beautifully." Overall, the feedback indicates strong performance and satisfaction with the product for IHC applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shashi (Verified Customer) (01-30-2020) | worked beautifully
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