GIYD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GIYD2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21158-1-AP

Synonyms

GIY YIG domain containing 2, GIYD1, GIYD2, SLX1

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 21158-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21158-1-AP (GIYD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 21158-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21158-1-AP (GIYD2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 21158-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21158-1-AP (GIYD2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

The immunogen of 21158-1-AP is GIYD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GIYD2 fusion protein Ag13094
• Full Name: GIY-YIG domain containing 2
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC000803
• Gene Symbol: GIYD2
• Gene ID (NCBI): 79008
• RRID: AB_2752255
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint DNA molecule resolution; Authors - Kaima Tsukada
human		Mol Cell; SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance.; Authors - Stephanie Panier
mouse	WB	Genes Dev; Break-induced replication promotes fragile telomere formation.; Authors - Zhe Yang ; KO Validated
human	WB	EMBO J; ATR limits Rad18-mediated PCNA monoubiquitination to preserve replication fork and telomerase-independent telomere stability; Authors - Siyuan Chen ; KD Validated