Connexin-43 Polyclonal antibody

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  WB analysis of HeLa using 15386-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15386-1-AP (GJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15386-1-AP

  IP result of anti-Connexin-43 (IP:15386-1-AP, 4ug; Detection:15386-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of mouse heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse heart using 15386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 15386-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Connexin-43 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 15386-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Synonyms

GJA1, Connexin 43, GJAL, Gap junction alpha-1 protein, Gap junction alpha 1 protein

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse heart tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 4 publications below
• IF: See 17 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

15386-1-AP targets Connexin-43 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6698

  Product name: Recombinant human Connexin 43 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-382 aa of BC026329

  Sequence: MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

• Full Name: gap junction protein, alpha 1, 43kDa
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-47 kDa
• GenBank Accession Number: BC026329
• Gene Symbol: Connexin-43
• Gene ID (NCBI): 2697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17302
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Connexin-43 (Cx43, also known as gap junction alpha-1), a connexin family member, plays an essential role in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation.; Authors - Yin Wang
mouse	IF	Thromb Res; Selective increase of cardiomyocyte derived extracellular vesicles after experimental myocardial infarction and functional effects on the endothelium.; Authors - Jose A Rodriguez
mouse	WB	Hypertension; Novel Role for the Immunoproteasome Subunit PSMB10 in Angiotensin II-Induced Atrial Fibrillation in Mice.; Authors - Jing Li
	IF	Ecotoxicol Environ Saf; Sodium Selenite inhibits mitophagy, downregulation and mislocalization of blood-testis barrier proteins of bovine Sertoli cell exposed to microcystin-leucine arginine (MC-LR) via TLR4/NF-kB and mitochondrial signaling pathways blockage.; Authors - E O Adegoke
human	WB	Environ Toxicol Pharmacol; Microcystin-leucine arginine (MC-LR) induced inflammatory response in bovine sertoli cell via TLR4/NF-kB signaling pathway.; Authors - E O Adegoke
human	IF	Free Radic Biol Med; Oxidative stress contributes to hepatocyte growth factor-dependent pro-senescence activity of ovarian cancer cells.; Authors - Justyna Mikuła-Pietrasik