GK2 Polyclonal antibody

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  WB analysis using 12913-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12913-1-AP (GK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

GK2 Polyclonal Antibody for WB, ELISA

Cat No. 12913-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GK 2, GK2, GKP2, GKTA, Glycerokinase 2, glycerol kinase 2

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12913-1-AP targets GK2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4010

  Product name: Recombinant human GK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 21-324 aa of BC029820

  Sequence: NSTRFLVFNSKTAELLSHHKVELTQEFPKEGWVEQDPKEILQSVYECIARTCEKLDELNIDISNIKAVGVSNQRETTVIWDKLTGEPLYNAVVWLDLRTQTTVEDLSKKIPGNSNFVKSKTGLPLSTYFSAVKLRWMLDNVRNVQKAVEEGRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNIHSLEWDKELCDFFEIPMDLLPNVFSSSEIYGLIKTGALEGVPISGCLGDQCAALVGQMCFQEGQAKNTYGTGCFLLCNTGRKCVFSEHGLLTTVAYKLGREKPAYYALEGSVAI

• Full Name: glycerol kinase 2
• Calculated Molecular Weight: 553 aa, 61 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC029820
• Gene Symbol: GK2
• Gene ID (NCBI): 2712
• RRID: AB_3669150
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14410
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GK2 (glycerol kinase 2) is a key enzyme in the regulation of glycerol uptake and metabolism.