GK2 Polyclonal antibody

GK2 Polyclonal Antibody for WB, ELISA

Cat No. 12913-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GK 2, GK2, GKP2, GKTA, Glycerokinase 2, glycerol kinase 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis using 12913-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12913-1-AP (GK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

mouse brain tissue, rat brain tissue

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

12913-1-AP targets GK2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4010 Product name: Recombinant human GK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-324 aa of BC029820 Sequence: NSTRFLVFNSKTAELLSHHKVELTQEFPKEGWVEQDPKEILQSVYECIARTCEKLDELNIDISNIKAVGVSNQRETTVIWDKLTGEPLYNAVVWLDLRTQTTVEDLSKKIPGNSNFVKSKTGLPLSTYFSAVKLRWMLDNVRNVQKAVEEGRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNIHSLEWDKELCDFFEIPMDLLPNVFSSSEIYGLIKTGALEGVPISGCLGDQCAALVGQMCFQEGQAKNTYGTGCFLLCNTGRKCVFSEHGLLTTVAYKLGREKPAYYALEGSVAI Predict reactive species
Full Name	glycerol kinase 2
Calculated Molecular Weight	553 aa, 61 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC029820
Gene Symbol	GK2
Gene ID (NCBI)	2712
RRID	AB_3669150
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14410
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

GK2 (glycerol kinase 2) is a key enzyme in the regulation of glycerol uptake and metabolism.

Product Specific Protocols
WB protocol for GK2 antibody 12913-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
mouse	WB	Mol Ther METTL5 deficiency induces oligoasthenoteratozoospermia via impaired 18S rRNA m(6)A methylation in humans and mice. Authors - Mengya Zhang View Article