Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13457-1-AP targets GK5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4269 Product name: Recombinant human GK5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC032470 Sequence: MSGLLTDPEQRAQEPRYPGFVLGLDVGSSVIRCHVYDRAARVCGSSVQKVENLYPQIGWVEIDPDVLWIQFVAVIKEAVKAAGIQMNQIVGLGISTQRATFITWNKKTGNHFHNFISWQDLRAVELVKSWNNSLLMKIFHSSCRVLHFFTRSKRLFTASLFTFTTQQTSLRLVWILQNLTEVQKAVEEENCCFGTIDTWLLYKLTKGSVYATDFSNASTTGLFDPYKMCWSGMITSLISIPLSLLPPVRDTSHNFGSVDEEIFGVPIPIVALLKVPGYDQNICYIFGKGTIEPVFYHGSTL Predict reactive species |
| Full Name | glycerol kinase 5 (putative) |
| Calculated Molecular Weight | 529 aa, 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC032470 |
| Gene Symbol | GK5 |
| Gene ID (NCBI) | 256356 |
| RRID | AB_2111453 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZS86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GK5(Putative glycerol kinase 5) is also named as ATP:glycerol 3-phosphotransferase 5 and belongs to the FGGY kinase family.It is involved in Glycerol metabolism and catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate.It has 3 isoforms produced by alternative splicing with the MW of 59 kDa, 34 kDa and 28 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GK5 antibody 13457-1-AP | Download protocol |
| WB protocol for GK5 antibody 13457-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Glycerol kinase 5 (GK5) antibody, catalog number 13457-1-AP, has 2 total citations. These appear in Journal of Experimental & Clinical Cancer Research and Clinical and Experimental Pharmacology and Physiology. The research fields include cancer drug resistance (gefitinib resistance via SREBP1/SCD1 signaling) and lipid metabolism (lipid droplet synthesis induced by particulate matter and cigarette smoke exposure).
| Species | Application | Title |
|---|---|---|
Clin Exp Pharmacol Physiol Exposure to particulate matter 2.5 and cigarette smoke induces the synthesis of lipid droplets by glycerol kinase 5. |
