GLI1 Polyclonal antibody

GLI1 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 25733-1-AP

Synonyms

GLI, GLI family zinc finger 1, GLI1, Glioma associated oncogene, Oncogene GLI, Zinc finger protein GLI1

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Validation Data Gallery

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  IHC staining of human gliomas using 25733-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25733-1-AP (GLI1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 25733-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25733-1-AP (GLI1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25733-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25733-1-AP (GLI1 Antibody) at dilution of 1:50 (under 10x lens).

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25733-1-AP is GLI1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLI1 fusion protein Ag22962
• Full Name: GLI family zinc finger 1
• Calculated Molecular Weight: 1106 aa, 118 kDa
• GenBank Accession Number: BC013000
• Gene Symbol: GLI1
• Gene ID (NCBI): 2735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Lab Chip; Paper-based microreactor array for rapid screening of cell signaling cascades.; Authors - Chia-Hao Huang
human	IF	Oncotarget; Investigating the role of Hedgehog/GLI1 signaling in glioblastoma cell response to temozolomide.; Authors - Jilian R Melamed
human	WB	Hum Cell; Retraction Note: Long noncoding RNA LINC00460 promotes the progression of cervical cancer via regulation of the miR-361-3p/Gli1 axis; Authors - Fan Li
human	WB	Oncol Rep; Combined inhibition of sonic Hedgehog signaling and histone deacetylase is an effective treatment for liver cancer.; Authors - Jingmin Li
human	WB	Am J Transl Res; Involvement of hedgehog signaling in all-trans retinoic acid-mediated suppression of colon cancer; Authors - Yu Xu
human,mouse	WB	J Biomater Appl; Alginate microspheres encapsulating hox transcript antisense RNA siRNA regulate the Hedgehog-Gli1 pathway to alleviate epidermal growth factor receptor tyrosine kinase inhibitors resistance; Authors - Guojie Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joshua (Verified Customer) (12-20-2018)

Cells were fixed in 4% paraformaldehyde and stained overnight at 4C. Cells were counterstained with phalliodin and DAPI. Staining is mix of weak nuclear and punctate cytosolic.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: U87 Human Glioblastoma

GLI1 Antibody Immunofluorescence, validation (1:500 dilution) in U87 Human Glioblastoma (Cat no:25733-1-AP)