Tested Applications
| Positive WB detected in | Jurkat cells, PC-3 cells, SH-SY5Y cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16551-1-AP targets GLI4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9497 Product name: Recombinant human GLI4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC014165 Sequence: MAALGDIQESPSVPSPVSLSSPGTPGTQHHEPQLHLHGHQHGSPGSSPKVLSQPSDLDLQDVEEVEIGRDTFWPDSEPKPEQAPRSPGSQAPDEGAGGALRSLLRSLPRRARCSAGFGPESSAERPAGQPPGAVPCAQPRGAWRVTLVQQAAAGPEGAPERAAELGVNFGRSRQGSARGAKPHRCEACGKSFKYNSLLLKHQRIHTGEKPYACHECGKRFRGWSGFIQHHRIHTGEKPYECGQCGRAFSHSSHFTQHLRIHNGEKPYKCGECGQAFSQSSNLVRHQRLHTGEKPYACSQCGKAFIWSSVLIEHQRIHTGEKPYECSDCGKAFRGRSHFFRHLRTHTGEKPFACGACGKAFGQSSQLIQHQRVHYRE Predict reactive species |
| Full Name | GLI family zinc finger 4 |
| Calculated Molecular Weight | 376 aa, 41 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC014165 |
| Gene Symbol | GLI4 |
| Gene ID (NCBI) | 2738 |
| RRID | AB_2109716 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10075 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GLI4 antibody 16551-1-AP | Download protocol |
| WB protocol for GLI4 antibody 16551-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this antibody reports use in Western Blot on fallopian tube epithelial cells at a 1:500 dilution with overnight incubation. The reviewer rated it 3 out of 5, noting that it works at the stated dilution. Overall, the feedback is neutral, confirming functional performance in WB without additional commentary on specificity or signal quality.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
iru (Verified Customer) (12-14-2020) | It works at 1:500 O/N incubation.
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