GLIS1 Polyclonal antibody

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  WB analysis of Jurkat using 23138-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23138-1-AP (GLIS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23138-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23138-1-AP (GLIS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 23138-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 23138-1-AP (GLIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 23138-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 23138-1-AP (GLIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GLIS1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23138-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

GLI similar 1, GLIS family zinc finger 1, GLI-similar 1, Zinc finger protein GLIS1

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

23138-1-AP targets GLIS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19214

  Product name: Recombinant human GLIS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 360-620 aa of BC101799

  Sequence: GPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPPAHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPTLPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHSLSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDT

• Full Name: GLIS family zinc finger 1
• Calculated Molecular Weight: 620 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC101799
• Gene Symbol: GLIS1
• Gene ID (NCBI): 148979
• RRID: AB_2879216
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q8NBF1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLIS1 is a nuclear zinc finger transcription factor that integrates developmental signaling and transcriptional control during neural and epithelial differentiation. It enhances cellular reprogramming efficiency and modulates pathways linked to Hedgehog and Wnt signaling. Dysregulated GLIS1 expression contributes to cardiac malformations, cancer progression, and stemness reactivation, highlighting its dual role in developmental regulation and disease pathogenesis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Reprod Sci; Effect of GLIS1 on the Lymph Node Metastasis of Cervical Squamous Carcinoma Based on Transcriptome Analysis; Authors - Jiazhen Huang
human	WB,IHC	BMC Biol; N6-methyladenosine regulates metabolic remodeling in kidney aging through transcriptional regulator GLIS1; Authors - Li Xu