GLIS1 Recombinant monoclonal antibody

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  WB analysis using 86902-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86902-1-RR (GLIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86902-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86902-1-RR against Human GLIS1 were performed. The affinity constant is 0.465 nM.

GLIS1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86902-1-RR

Clone No.251891F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GLI similar 1, GLIS family zinc finger 1, GLI-similar 1, Zinc finger protein GLIS1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86902-1-RR targets GLIS1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag40019

  Product name: Recombinant human GLIS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 360-620 aa of BC101799

  Sequence: GPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPPAHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPTLPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHSLSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDT

• Full Name: GLIS family zinc finger 1
• Calculated Molecular Weight: 620 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC101799
• Gene Symbol: GLIS1
• Gene ID (NCBI): 148979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NBF1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLIS1 is a nuclear zinc finger transcription factor that integrates developmental signaling and transcriptional control during neural and epithelial differentiation. It enhances cellular reprogramming efficiency and modulates pathways linked to Hedgehog and Wnt signaling. Dysregulated GLIS1 expression contributes to cardiac malformations, cancer progression, and stemness reactivation, highlighting its dual role in developmental regulation and disease pathogenesis.