GLRA1 Polyclonal antibody

GLRA1 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17951-1-AP

Synonyms

GLRA1, glycine receptor, alpha 1, STHE



Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, SH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17951-1-AP targets GLRA1 in WB, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLRA1 fusion protein Ag12431
Full Name glycine receptor, alpha 1
Calculated molecular weight 449 aa, 52 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC074980
Gene symbol GLRA1
Gene ID (NCBI) 2741
RRIDAB_2110060
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Protocols

Product Specific Protocols
WB protocol for GLRA1 antibody 17951-1-APDownload protocol
FC protocol for GLRA1 antibody 17951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseCoIP

PLoS Biol

mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.

Authors - Zi-Yang Zhang
mouseWB,IF

J Biol Chem

Clinical, genetic, and functional characterization of the glycine receptor β-subunit A455P variant in a family affected by hyperekplexia syndrome.

Authors - Ghada I Aboheimed
mouseWB

Psychopharmacology (Berl)

Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression.

Authors - Niannian Huang
mouseWB

Eur J Pharmacol

Functional expression of glycine receptors in DRG neurons of mice.

Authors - Lin Yao
humanWB

Neuropharmacology

Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.

Authors - Zi-Yang Zhang