|Positive WB detected in||mouse brain tissue, mouse cerebellum tissue, SH-SY5Y cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Sample-dependent, check data in validation data gallery|
17951-1-AP targets GLRA1 in WB, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GLRA1 fusion protein Ag12431|
|Full Name||glycine receptor, alpha 1|
|Calculated molecular weight||449 aa, 52 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC074980|
|Gene ID (NCBI)||2741|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)
Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.
mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.
Eur J Pharmacol
Functional expression of glycine receptors in DRG neurons of mice.
Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression.