GLRA1 Polyclonal antibody

GLRA1 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 17951-1-AP

Synonyms

GLRA1, glycine receptor, alpha 1, STHE



Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, SH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Product Information

17951-1-AP targets GLRA1 in WB, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLRA1 fusion protein Ag12431
Full Name glycine receptor, alpha 1
Calculated molecular weight 449 aa, 52 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC074980
Gene symbol GLRA1
Gene ID (NCBI) 2741
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Protocols

Product Specific Protocols
WB protocol for GLRA1 antibody 17951-1-APDownload protocol
FC protocol for GLRA1 antibody 17951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Neuropharmacology

Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.

Authors - Zi-Yang Zhang
mouseCoIP

PLoS Biol

mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.

Authors - Zi-Yang Zhang
mouseWB

Eur J Pharmacol

Functional expression of glycine receptors in DRG neurons of mice.

Authors - Lin Yao
mouseWB

Psychopharmacology (Berl)

Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression.

Authors - Niannian Huang