Tested Applications
| Positive WB detected in | MCF7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13145-1-AP targets GLRA3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3702 Product name: Recombinant human GLRA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 29-255 aa of BC036086 Sequence: KETDSARSRSAPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSIAETTMDYRVNIFLRQKWNDPRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGANFHEVTTDNKLLRIFKNGNVLYSIRLTLTLSCPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQDEAPVQVAEGLTLPQFLLKEEKDLRYCTKHYNTGKFTCIEVRFHLERQMGY Predict reactive species |
| Full Name | glycine receptor, alpha 3 |
| Calculated Molecular Weight | 464 aa, 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC036086 |
| Gene Symbol | GLRA3 |
| Gene ID (NCBI) | 8001 |
| RRID | AB_10638905 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75311 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GLRA3 antibody 13145-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GLRA3 Polyclonal antibody (Cat# 13145-1-AP) has 2 citations. They appear in PLoS Biology and Neuropharmacology, both by Zi-Yang Zhang (2019). The research fields involve glycine receptor signaling in the spinal dorsal horn, specifically mGluR5/ERK-mediated phosphorylation of the GlyR α1 subunit in mice and HUWE1-mediated ubiquitination of the glycine receptor in human spinal cord. Both studies utilized the antibody for Western blot.
| Species | Application | Title |
|---|---|---|
PLoS Biol mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice. | ||
Neuropharmacology Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn. |
Reviews
What customers say
The single review for this product rates it 4 out of 5 stars. The user performed a Western blot on lumbar spinal cord samples at a 1:1000 dilution and observed a mild band for the alpha 3 target. Overall, the feedback suggests the antibody works but may benefit from optimization to achieve stronger signal intensity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Braulio (Verified Customer) (02-26-2020) | Western blot in lumbar spinal cord samples, shows a mild band for alpha 3
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