|Positive WB detected in||MCF7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
13145-1-AP targets GLRA3 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GLRA3 fusion protein Ag3702|
|Full Name||glycine receptor, alpha 3|
|Calculated molecular weight||464 aa, 54 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC036086|
|Gene ID (NCBI)||8001|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)
Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.
mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Braulio (Verified Customer) (02-26-2020)
Western blot in lumbar spinal cord samples, shows a mild band for alpha 3