GLT25D1 Polyclonal antibody

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• GLT25D1 Polyclonal antibody (16768-1-AP)

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• KD/KO Validated

GLT25D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16768-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PSEC0241, EC:2.4.1.50, Collagen beta(1-O)galactosyltransferase 1, COLGALT1, ColGalT 1

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  WB analysis of SH-SY5Y using 16768-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SH-SY5Y using 16768-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SH-SY5Y using 16768-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16768-1-AP (GLT25D1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 16768-1-AP

  IP result of anti-GLT25D1 (IP:16768-1-AP, 4ug; Detection:16768-1-AP 1:500) with HeLa cells lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 16768-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16768-1-AP (GLT25D1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse placenta using 16768-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 16768-1-AP (GLT25D1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of SH-SY5Y using 16768-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 16768-1-AP (GLT25D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of SH-SY5Y using 16768-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 16768-1-AP (GLT25D1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, mouse placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16768-1-AP targets GLT25D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10346

  Product name: Recombinant human GLT25D1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-231 aa of BC020492

  Sequence: MLPGYRDPYHGRPLTKGELGCFLSHYNIWKEVVDRGLQKSLVFEDDLRFEIFFKRRLMNLMRDVEREGLDWDLIYVGRKRMQVEHPEKAVPRVRNLVEADYSYWTLAYVISLQGARKLLAAEPLSKMLPVDEFLPVMFDKHPVSEYKAHFSLRNLHAFSVEPLLIYPTHYTGDDGYVSDTETSVVWNNEHVKTDWDRAKSQKMREQQALSREAKNSDVLQSPLDSAARDEL

• Full Name: glycosyltransferase 25 domain containing 1
• Calculated Molecular Weight: 231 aa, 27 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC020492
• Gene Symbol: GLT25D1
• Gene ID (NCBI): 79709
• RRID: AB_2878313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NBJ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Ann Neurol; Biallelic COLGALT1 variants are associated with cerebral small vessel disease.; Authors - Satoko Miyatake
mouse,rat	WB	J Mol Cell Cardiol; Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.; Authors - Randy T Cowling
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
mouse	WB	Sci Rep; Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes; Authors - Masahiko Terajima
human	WB	Biochemistry; Role of Glycosyltransferase 25 domain 1 in Type I Collagen Glycosylation and Molecular Phenotypes.; Authors - Masahiko Terajima ; KD Validated
mouse	WB,IHC	Mol Med Rep; Downregulation of Glt25d1 aggravates carbon tetrachloride‑induced acute hepatic injury through activation of the TGF‑β1/Smad2 signaling pathway.; Authors - Xiaohui Ye ; KO Validated