Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16768-1-AP targets GLT25D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10346 Product name: Recombinant human GLT25D1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-231 aa of BC020492 Sequence: MLPGYRDPYHGRPLTKGELGCFLSHYNIWKEVVDRGLQKSLVFEDDLRFEIFFKRRLMNLMRDVEREGLDWDLIYVGRKRMQVEHPEKAVPRVRNLVEADYSYWTLAYVISLQGARKLLAAEPLSKMLPVDEFLPVMFDKHPVSEYKAHFSLRNLHAFSVEPLLIYPTHYTGDDGYVSDTETSVVWNNEHVKTDWDRAKSQKMREQQALSREAKNSDVLQSPLDSAARDEL Predict reactive species |
| Full Name | glycosyltransferase 25 domain containing 1 |
| Calculated Molecular Weight | 231 aa, 27 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | BC020492 |
| Gene Symbol | GLT25D1 |
| Gene ID (NCBI) | 79709 |
| RRID | AB_2878313 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NBJ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| IHC protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| IP protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| WB protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GLT25D1 Polyclonal antibody (Cat# 16768-1-AP) has 11 citations across 10 journals, including Nature Communications, Annals of Neurology, Cancer Cell International, Molecular Medicine Reports, Journal of Molecular and Cellular Cardiology, Scientific Reports, European Journal of Medical Research, PLoS Genetics, Disease Models & Mechanisms, and Biochemistry. Research fields span collagen post-translational modification and glycosylation, cerebral small vessel disease, hepatocellular carcinoma, acute hepatic injury, cardiac fibrosis, renal cell carcinoma, skin collagen biology, and musculoskeletal development.
| Species | Application | Title |
|---|---|---|
Nat Commun The structural basis for the human procollagen lysine hydroxylation and dual-glycosylation | ||
Ann Neurol Biallelic COLGALT1 variants are associated with cerebral small vessel disease. | ||
Cancer Cell Int The collagen-modifying enzyme GLT25D1 is a prognostic indicator related to immunosuppression and malignant phenotypes in hepatocellular carcinoma | ||
Mol Med Rep Downregulation of Glt25d1 aggravates carbon tetrachloride‑induced acute hepatic injury through activation of the TGF‑β1/Smad2 signaling pathway.
| ||
Sci Rep Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes |
