GLT25D2 Polyclonal antibody

GLT25D2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25993-1-AP

Synonyms

EC:2.4.1.50, Collagen beta(1-O)galactosyltransferase 2, COLGALT2, ColGalT 2, C1orf17

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 25993-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25993-1-AP (GLT25D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 25993-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25993-1-AP (GLT25D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 25993-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25993-1-AP (GLT25D2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 25993-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25993-1-AP (GLT25D2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, Neuro-2a cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25993-1-AP targets GLT25D2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLT25D2 fusion protein Ag23360
• Full Name: glycosyltransferase 25 domain containing 2
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC035672
• Gene Symbol: GLT25D2
• Gene ID (NCBI): 23127
• RRID: AB_2880324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Dis Model Mech; Loss of function of Colgalt1 disrupts collagen post-translational modification and causes musculoskeletal defects.; Authors - Krista A Geister