Tested Applications
| Positive WB detected in | HeLa cells, mouse pancreas tissue |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
27353-1-AP targets GLTSCR2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26358 Product name: Recombinant human GLTSCR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 141-440 aa of BC010095 Sequence: NAKKLRRKEQLWEKLAKQGELPREVRRAQARLLNPSATRAKPGPQDTVERPFYDLWASDNPLDRPLVGQDEFFLEQTKKKGVKRPARLHTKPSQAPAVEVAPAGASYNPSFEDHQTLLSAAHEVELQRQKEAEKLERQLALPATEQAATQESTFQELCEGLLEESDGEGEPGQGEGPEAGDAEVCPTPARLATTEKKTEQQRRREKAVHRLRVQQAALRAARLRHQELFRLRGIKAQVALRLAELARRRRRRQARREAEADKPRRLGRLKYQAPDIDVQLSSELTDSLRTLKPEGNILRD Predict reactive species |
| Full Name | glioma tumor suppressor candidate region gene 2 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC010095 |
| Gene Symbol | GLTSCR2 |
| Gene ID (NCBI) | 29997 |
| RRID | AB_2880852 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZM5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GLTSCR2 antibody 27353-1-AP | Download protocol |
| WB protocol for GLTSCR2 antibody 27353-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun MTR4 adaptor PICT1 functions in two distinct steps during pre-rRNA processing
| ||
Dev Cell Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method | ||
Biochem Biophys Res Commun Pre-ribosomal WDR74 module coordinates the early and late pre-rRNA processing stages for the NVL2-mediated regulation of 60S ribosome biogenesis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Elisa (Verified Customer) (03-15-2022) | U2OS cells were fixed in 4% PFA for 15' at Room temperature. Cells were washed once with PBS for. Membrane permeabilisation was then performed with 0.3% Triton in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at room temperature. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). In this picture, cells are stained for Hoechst (DNA marker, in magenta) and GLTSCR2 (nucleolar protein in yellow).
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