GLUD2 Polyclonal antibody

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GLUD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14462-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GLUDP1, GLUD 2, GDH2, GDH 2, EC:1.4.1.3

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  WB analysis using 14462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14462-1-AP (GLUD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14462-1-AP

  WB result of GLUD2 antibody (14462-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLUD2 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 14462-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 14462-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 14462-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GLUD2 antibody (14462-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HeLa cells, HepG2 cells，U-251 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14462-1-AP targets GLUD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5748

  Product name: Recombinant human GLUD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 207-558 aa of BC050732

  Sequence: MELAKKGFIGPGVDVPAPDMNTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFRDKTFVVQGFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKAKPYEGSILEVDCDILIPAATEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLERNILVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLLSVQESLERKFGKHGGTIPIVPTAEFQDSISGASEKDIVHSALAYTMERSARQIMHTAMKYNLGLDLRTAAYVNAIEKVFKVYSEAGVTFT

• Full Name: glutamate dehydrogenase 2
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC050732
• Gene Symbol: GLUD2
• Gene ID (NCBI): 2747
• RRID: AB_2110642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49448
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The GLUD2 Polyclonal antibody (Cat# 14462-1-AP) has 3 citations published in Nature Metabolism, Translational Oncology, and Amino Acids. The research fields span gut microbiome–host stress interactions, quantitative proteomic profiling of hepatocellular carcinoma, and proline metabolic reprogramming in lung adenocarcinoma. Applications cited include Western blot and immunohistochemistry in human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang
human	IHC	Transl Oncol; Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level.; Authors - Xuyong Wei
human	WB	Amino Acids; PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.; Authors - Chunhong Cui