Anti-Human GM-CSF Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98050-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

  1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG(red). 1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98050-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98050-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98050-1-RR against Human GM-CSF were performed. The affinity constant is 3.46 nM.

GM-CSF Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98050-1-PBS

Clone No.240183C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CSF2, 240183C7, Colony stimulating factor, Colony Stimulating Factor 2, Colony-stimulating factor

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Product Information

98050-1-PBS targets GM-CSF in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0189

  Product name: Recombinant Human GM-CSF protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: N-6*His

  Domain: 18-144 aa of

  Sequence: APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

• Full Name: colony stimulating factor 2 (granulocyte-macrophage)
• Gene Symbol: GM-CSF
• Gene ID (NCBI): 1437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P04141
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).