GMIP Polyclonal antibody

GMIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25917-1-AP

Synonyms

GEM-interacting protein, GEM interacting protein, ARHGAP46

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Validation Data Gallery

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  WB analysis using 25917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25917-1-AP (GMIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25917-1-AP

  IP result of anti-GMIP (IP:25917-1-AP, 4ug; Detection:25917-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human ovary cancer using 25917-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25917-1-AP (GMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 25917-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25917-1-AP (GMIP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 25917-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GMIP antibody (25917-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 25917-1-AP

  Immunofluorescent analysis of HepG2 cells using 25917-1-AP (GMIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human stomach cancer tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

25917-1-AP targets GMIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GMIP fusion protein Ag23239
• Full Name: GEM interacting protein
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC126436
• Gene Symbol: GMIP
• Gene ID (NCBI): 51291
• RRID: AB_2880295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo.

Publications

Species	Application	Title
human	WB,IF	Int Immunopharmacol; Identification of TAP1 as a T-cell related therapeutic target in gastric cancer by mediating oxalipliatin-related synergistic enhancement of immunotherapy; Authors - Yupeng Zhao