GMPPA Polyclonal antibody

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  WB analysis using 15517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15517-1-AP (GMPPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15517-1-AP

  IP result of anti-GMPPA (IP:15517-1-AP, 4ug; Detection:15517-1-AP 1:500) with HEK-293 cells lysate 2600ug.

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  IHC staining of human stomach cancer using 15517-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15517-1-AP (GMPPA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

GMPPA Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15517-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Mannose-1-phosphate guanyltransferase alpha, GTP-mannose-1-phosphate guanylyltransferase alpha, GMPP-alpha, GDP-mannose pyrophosphorylase A, GDP mannose pyrophosphorylase A

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

15517-1-AP targets GMPPA in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7789

  Product name: Recombinant human GMPPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-420 aa of BC007456

  Sequence: MLKAVILIGGPQKGTRFRPLSFEVPKPLFPVAGVPMIQHHIEACAQVPGMQEILLIGFYQPDEPLTQFLEAAQQEFNLPVRYLQEFAPLGTGGGLYHFRDQILAGSPEAFFVLNADVCSDFPLSAMLEAHRRQRHPFLLLGTTANRTQSLNYGCIVENPQTHEVLHYVEKPSTFISDIINCGIYLFSPEALKPLRDVFQRNQQDGQLEDSPGLWPGAGTIRLEQDVFSALAGQGQIYVHLTDGIWSQIKSAGSALYASRLYLSRYQDTHPERLAKHTPGGPWIRGNVYIHPTAKVAPSAVLGPNVSIGKGVTVGEGVRLRESIVLHGATLQEHTCVLHSIVGWGSTVGRWARVEGTPSDPNPNDPRARMDSESLFKDGKLLPAITILGCRVRIPAEVLILNSIVLPHKELSRSFTNQIIL

• Full Name: GDP-mannose pyrophosphorylase A
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC007456
• Gene Symbol: GMPPA
• Gene ID (NCBI): 29926
• RRID: AB_2111056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96IJ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	iScience; A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation.; Authors - Norio Sasaoka
mouse	WB	Front Mol Biosci; Altered Glycosylation in the Aging Heart.; Authors - Patricia Franzka
human,mouse	WB	Front Nutr; Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12; Authors - Patricia Franzka
mouse	WB	Biomedicines; Impact of Hypermannosylation on the Structure and Functionality of the ER and the Golgi Complex; Authors - Patricia Franzka ; KO Validated
human	WB	Front Mol Neurosci; Ubiquitination contributes to the regulation of GDP-mannose pyrophosphorylase B activity; Authors - Patricia Franzka
mouse	WB	J Clin Invest; GMPPA defects cause a neuromuscular disorder with α-dystroglycan hyperglycosylation; Authors - Patricia Franzka