GNA15 Polyclonal antibody, PBS Only

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• GNA15 Polyclonal antibody, PBS Only (12078-1-PBS)

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  WB analysis using 12078-1-AP (same clone as 12078-1-PBS)

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 12078-1-AP (GNA15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12078-1-PBS in a different storage buffer formulation.

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  WB analysis using 12078-1-AP (same clone as 12078-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12078-1-AP (GNA15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12078-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 12078-1-AP (same clone as 12078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12078-1-AP (GNA15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12078-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 12078-1-AP (same clone as 12078-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12078-1-AP (GNA15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12078-1-PBS in a different storage buffer formulation.

GNA15 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12078-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

12078-1-PBS targets GNA15 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2738

  Product name: Recombinant human GNA15 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-374 aa of BC013585

  Sequence: MARSLTWRCCPWCLTEDEKAAARVDQEINRILLEQKKQDRGELKLLLLGPGESGKSTFIKQMRIIHGAGYSEEERKGFRPLVYQNIFVSMRAMIEAMERLQIPFSRPESKHHASLVMSQDPYKVTTFEKRYAAAMQWLWRDAGIRACYERRREFHLLDSAVYYLSHLERITEEGYVPTAQDVLRSRMPTTGINEYCFSVQKTNLRIVDVGGQKSERKKWIHCFENVIALIYLASLSEYDQCLEENNQENRMKESLALFGTILELPWFKSTSVILFLNKTDILEEKIPTSHLATYFPSFQGPKQDAEAAKRFILDMYTRMYTGCVDGPEGSKKGARSRRLFSHYTCATDTQNIRKVFKDVRDSVLARYLDEINLL

• Full Name: guanine nucleotide binding protein (G protein), alpha 15 (Gq class)
• Calculated Molecular Weight: 374 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC013585
• Gene Symbol: GNA15
• Gene ID (NCBI): 2769
• RRID: AB_3085389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30679
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GNA15, also known as GNA16, belongs to the G-alpha family and G(q) subfamily. The protein encoded by the GNA15 gene is the alpha subunit of the G protein, which participates in the regulation of cell proliferation and apoptosis. GNA15 is highly expressed in some cell types, such as hematopoietic stem cells and epithelial cells during specific stages of differentiation. GNA15 is known to play as an oncogene in several tumors, such as gastroenteropancreatic neuroendocrine neoplasia (GEP-NEN), liver cancer, pancreatic ductal adenocarcinoma and ovarian cancer (PMID: 34552875). The molecular weight of GNA15 is 44 kDa.